Porous Substrate Plates And The Use Thereof

• US 20100323915A1
• Filed: 08/11/2010
• Published: 12/23/2010
• Est. Priority Date: 04/12/2004
• Status: Active Grant

• Current Assignee: Ann M. Ferrie, Brian L. Webb, Ye Fang, Yulong Hong
• Original Assignee: Ann M. Ferrie, Brian L. Webb, Ye Fang, Yulong Hong
• Inventors: Hong, Yulong, Ferrie, Ann M., Webb, Brian L., Fang, Ye
• Granted Patent: US 8,129,198 B2
• Time in Patent Office: Days
• US Class Current: 506/9
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2300/069 Absorbents; Gels to retain a fluid
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: Y10S 435/81 Packaged device or kit
• CPC Class Codes: Y10S 436/809 Multifield plates or multicontainer arrays
• CPC Class Codes: Y10T 29/49885 with coating before or during assembling

Abstract

A substrate plate or device adapted for use with biological or chemical assays is disclosed. The device may take the form of a multi-well plate having a three-dimensional, porous layer as part of a support surface within each well for immobilizing probe species. The porous layer is characterized as having a plurality of interconnected voids defined by a matrix of contiguous solid material. A method and its variants are also described.

Claims

1-14. -14. (canceled)

15. A method for manufacturing a microplate, the method comprises:

providing a glass support;

depositing a granular material onto said support to form a defined area of granular material;

adhering individual particles of said granular material together to form a porous layer of interconnected voids attached to said support;

providing a frame having a number of wells, each defined by at least a sidewall; and

assembling said frame with said support to construct a microplate.

16-19. -19. (canceled)

20. A method for manufacturing a microplate, the method comprises:

providing an understructure support of either a non-porous glass or polymer material;

depositing a polymeric granular material onto a surface of said support to form a defined area of polymeric granular material;

binding said polymeric granular material together to form a porous layer of interconnected voids attached to said support;

providing a frame having a number of wells; and

assembling said frame with said support.

21. A method of using a microplate, the method comprises:

providing a microplate having a number of wells, each of said wells having a three-dimensional porous-matrix located therein as a porous layer, said porous layer being either modified or unmodified with a predetermined surface chemistry for immobilizing probe species;

depositing biological probes at a number of defined locations on said porous layer; and

performing a bioassay with a sample.

22. The method according to claim 21, further comprising entrapping a portion of said probes in a portion of voids within said porous matrix when said porous layer is an unmodified, bare substrate.

23. The method according to claim 21, wherein said probes are deposited either as an array of a number of microspots or as a single spot with a diameter of greater than or equal to 100 micrometers.

24. The method according to claim 21, wherein said probes are selected from the group consisting of nucleic acids, membrane-proteins, proteins, carbohydrates, lipids, or chemical molecules.

25. The method according to claim 21, wherein said membrane-proteins are selected from GPCRs, ion-channels, tyrosine kinase receptors, immuno-receptors, and transporters.

26. The method according to claim 21, wherein when said probes are membrane proteins associated with lipid molecules, the porous substrate is uncoated with a material that modifies surface properties of said porous substrate.

27. The method according to claim 21, wherein the biological membrane is selected from any one of the following:

a cell-membrane fragment preparation, a lipid vesicle containing reconstituted membrane-protein, or a lipid micelle containing a membrane-protein, an exosome vesicle particle containing at least a membrane-protein of interest.