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GENOME ANALYSIS USING A NICKING ENDONUCLEASE

  • US 20100330556A1
  • Filed: 06/30/2009
  • Published: 12/30/2010
  • Est. Priority Date: 06/30/2009
  • Status: Abandoned Application
First Claim
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1. A method of sample analysis comprising:

  • a) contacting a genomic sample comprising a double-stranded DNA with a site-specific nicking endonuclease to provide a nicked double-stranded DNA comprising a plurality of nick sites, wherein said nicking endonuclease nicks a site adjacent to a variable nucleotide;

    b) contacting said nicked double-stranded DNA with a polymerase in the presence of a nucleotide composition comprising a first labeled chain terminator nucleotide comprising a first label, thereby producing a labeled double-stranded DNA in which not every nick site is labeled with said first label;

    c) stretching out said labeled double-stranded DNA to provide a stretched, labeled double-stranded DNA; and

    d) imaging said stretched, labeled double-stranded DNA to identify a labeling pattern on said stretched labeled double-stranded DNA.

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