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Hydroxymethyl Linkers For Labeling Nucleotides

  • US 20100330569A1
  • Filed: 04/21/2010
  • Published: 12/30/2010
  • Est. Priority Date: 04/23/2009
  • Status: Abandoned Application
First Claim
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1. A method of incorporating labeled nucleotides into nucleic acid, comprising:

  • a) providing a plurality of nucleic acid template molecules, a polymerase, and a plurality of nucleotide analogues wherein each nucleotide analogue is labeled with a unique label and contains a removable chemical moiety capping the 3′

    -OH group, wherein the label is attached to the base through a hydroxymethyl group;

    b) incorporating a first nucleotide analogue with said polymerase; and

    c) detecting the label of the incorporated nucleotide analogue.

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