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Method for Nutrient Pre-Loading of Microbial Cells

  • US 20100330653A1
  • Filed: 06/24/2009
  • Published: 12/30/2010
  • Est. Priority Date: 06/24/2009
  • Status: Abandoned Application
First Claim
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1. A method for growing selected microbial cells in a non-sterile system comprising the steps of:

  • providing a closed reactor for growing the selected microbial cells;

    preparing a nutrient mix for supporting a desired level of growth of the selected microbial cells, with said nutrient mix including a required amount of each nutrient necessary for the desired level of growth and a surplus amount of a chosen nutrient exceeding the respective required amount;

    feeding the nutrient mix into the closed reactor;

    growing the selected microbial cells in the closed reactor to the desired level, with the selected microbial cells storing the surplus amount of the chosen nutrient;

    eliminating the chosen nutrient from the non-sterile system; and

    transferring the selected microbial cells to the non-sterile system, where the selected microbial cells rely on the stored surplus amount of the chosen nutrient for growth.

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