ELECTRON BEAM NUCLEIC ACID SEQUENCING
First Claim
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1. A method for obtaining sequence information from nucleic acid molecules, comprising:
- scanning with an electron beam a population of labeled nucleic acid molecules on a substrate, wherein the nucleic acid molecules are deposited from a tip onto said substrate that is moving in a direction such that the nucleic acid molecules are elongated on the substrate and aligned along the direction of the movement, wherein said scanning detects said labels and thereby obtaining sequence information of said nucleic acid molecules.
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Abstract
The present invention relates to compositions, methods, and uses for obtaining sequence information from nucleic acid molecules.
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Citations
24 Claims
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1. A method for obtaining sequence information from nucleic acid molecules, comprising:
scanning with an electron beam a population of labeled nucleic acid molecules on a substrate, wherein the nucleic acid molecules are deposited from a tip onto said substrate that is moving in a direction such that the nucleic acid molecules are elongated on the substrate and aligned along the direction of the movement, wherein said scanning detects said labels and thereby obtaining sequence information of said nucleic acid molecules. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A method for obtaining sequence information from a nucleic acid molecule deposited on a substrate surface, wherein said molecule is elongated, comprising:
scanning said nucleic acid molecule with an electron beam, wherein one or more types of nucleotide bases of said nucleic acid molecule is labeled, said scanning yields a signal that is resolved via electron energy loss spectroscopy (EELS), and wherein said signal is indicative of the identity and order of said labeled nucleotide base within said nucleic acid molecule, thereby obtaining sequence information from said nucleic acid molecule. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
Specification