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METHODS AND SYSTEMS FOR MONITORING MULTIPLE OPTICAL SIGNALS FROM A SINGLE SOURCE

  • US 20100331212A1
  • Filed: 09/03/2010
  • Published: 12/30/2010
  • Est. Priority Date: 08/11/2005
  • Status: Abandoned Application
First Claim
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1. An optical analysis method comprising:

  • simultaneously providing excitation radiation over time to each of a plurality of confined sources on a substrate, wherein each confined source comprises a polymerase enzyme associated with a template nucleic acid sequence;

    exposing the polymerase enzymes to polymerase reaction conditions including two or more differently fluorescently labeled types of NTPs, whereby each differently labeled type of NTP has a different emission wavelength profile;

    passing the emitted light through an optical train such that for each confined source the optical signal from each type of NTP is directed along a different optical path than each other type of NTP, such that the optical signal from each type of NTP is directed to a different detector location;

    observing the optical signals over time from each confined source to determine a time sequence of NTP incorporation, thereby obtaining information about the template nucleic acid sequences.

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