MICROPARTICLES WITH ENHANCED COVALENT BINDING CAPACITY AND THEIR USES
First Claim
1. A polyelectrolyte which is immobilized on a surface and has multiple functional groups exposed for covalent binding to biomolecules.
7 Assignments
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Accused Products
Abstract
A polyelectrolyte having multiple exposed functional groups, each such group being capable of covalently bonding to a molecule, is immobilized on a surface for the purpose of bonding to a biomolecule. The biomolecule can be, for example, a nucleic acid, e.g., an amine functionalized oligonucleotide. The polyelectrolyte can include, e.g., BSA (Bovine Serum Albumin) which is bound to a functionalized surface using a covalent immobilization strategy, e.g., reaction with the surface of a tosyl-activated microparticle. Following such reaction, exposed reactive functional groups on the protein, such as amine, carboxyl, thiol, hydroxyl groups can further be utilized to covalently couple the oligonucleotide of interest using suitable chemistry.
100 Citations
16 Claims
- 1. A polyelectrolyte which is immobilized on a surface and has multiple functional groups exposed for covalent binding to biomolecules.
- 3. A product comprising a polyelectrolyte which is immobilized on a surface and covalently bound to a nucleic acid.
- 9. A process comprising covalently bonding a nucleic acid to a polyelectrolyte immobilized on a surface.
Specification