Method of generating a fluid stream in a microfluidic device
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Accused Products
Abstract
A method of continuously regulating external gas pressure on a variable volume container for the delivery of fluids to a microfluidic device and analysis of particles based upon relative presence or absence of at least one particle characteristic.
26 Citations
109 Claims
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1-90. -90. (canceled)
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91. A method of flow cytometry, comprising the steps of:
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a. providing a variable volume container having a flexible wall; b. establishing an amount of fluid in said variable volume container; c. exerting an amount of pressure with an amount of a gas on an exterior surface of said flexible wall of said variable volume container; d. providing a conduit between said variable volume container and a flow path of a flow cytometer; e. generating a fluid stream in said flow path of said flow cytometer; f. intermittently entraining a particle in said fluid stream in said flow path of said flow cytometer; g. generating oscillations in said fluid stream to establish a plurality of droplets; h. establishing said particle in one of said plurality of droplets; i. illuminating said particle in said one of said plurality of droplets for a duration of time; j. detecting an emission generated by said particle which varies based upon corresponding variation of at least one particle characteristic of said particle; and k. differentiating said particle based upon relative presence or absence of said at least one particle characteristic. - View Dependent Claims (92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109)
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Specification