STABILIZATION OF MACROMOLECULAR MEMBRANES

• US 20110002844A1
• Filed: 02/11/2009
• Published: 01/06/2011
• Est. Priority Date: 03/17/2008
• Status: Abandoned Application

• Current Assignee: Trustees Of The University Of Pennsylvania (University of Pennsylvania)
• Original Assignee: Trustees Of The University Of Pennsylvania (University of Pennsylvania)
• Inventors: Hammer, Daniel A., Burdick, Jason A., Katz, Joshua S.
• Application Number:
  US12/919,119
• Publication Number:
  US 20110002844A1
• Time in Patent Office: Days
• US Class Current: 424/1.21
• CPC Class Codes: A61K 31/337 having four-membered rings, e.g. taxol
• CPC Class Codes: A61K 9/1273 Polymersomes; Liposomes with polymerisable or polymerised bilayer-forming substances polymers grafted or coated on phosphatidyl liposomes A61K9/1271, on non-phosphatidyl liposomes A61K9/1272
• CPC Class Codes: A61K 9/5026 obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyvinyl pyrrolidone, poly(meth)acrylates
• CPC Class Codes: A61K 9/5031 obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyethylene glycol, poly(lactide-co-glycolide)
• CPC Class Codes: A61P 35/00 Antineoplastic agents

Abstract

Disclosed are stabilized polymersomes having layer structures. Such stabilized polymersomes are, in some embodiments, biocompatible, and are capable of enhanced, sustained release of agents. Also disclosed are related methods for synthesizing such stabilized polymersomes and methods for using such polymersomes for delivery of therapeutic, imaging, and various other agents.

Claims

1. A method of synthesizing a stabilized polymersome, comprising:

forming a polymersome, having a layer structure, from chains of multiblock copolymer comprising hydrophobic and hydrophilic blocks;

at least some of the hydrophobic blocks comprising one or more polymerizable groups; and

reacting two or more of the polymerizable groups to form covalent bonds between chains of the copolymer.

2. The method of claim 1, wherein the multiblock copolymer comprises a diblock copolymer, a triblock copolymer, or any combination thereof.

3. The method of claim 2, wherein the multiblock copolymer comprises poly(caprolactone)-poly(ethylene glycol).

4. The method of claim 1, wherein the reacting comprises reacting two or more of the polymerizable groups in the presence of an initiator.

5. The method of claim 1, wherein the reacting is effected by an environmental condition exterior to the multiblock copolymer.

6. The method of claim 1, wherein the layer structure comprises a bilayer.

7. The method of claim 6, wherein the reacting gives rise to covalent bonds between the individual polymers comprising the bilayer.

8. The method of claim 1, wherein the initiator is 2,2-dimethoxy-2-phenylacetophenone.

9. The method of claim 4, wherein reacting the polymerizable groups comprises exposing the polymerizable groups to ultraviolet light, heat, or both in the presence of the initiator.

10. The method of claim 1, wherein the polymerizable group comprises an acrylate, methacrylate, acrylamide, methacrylamide, vinyl, or vinyl sulfone unit.

11. The method of claim 1, further comprising disposing one or more agents within the stabilized polymersome, within the layer structure, on the surface of the polymersome, or any combination thereof.

12. The method of claim 11, wherein the agent comprises a therapeutic composition, an imaging composition, a binding composition, or any combination thereof.

13. The method of claim 1, wherein the stabilized polymersome is characterized as being biodegradable.

14. The method of claim 1, wherein a majority of the hydrophobic blocks bear a polymerizable group.

15. The method of claim 1, further comprising forming one or more cross-links between multiblock copolymer chains.

16. The method of claim 15, wherein the cross-links are formed by introducing a composition having multiple polymerizable groups to the chains of multiblock copolymer.

17. The method of claim 15, wherein the multiblock copolymer comprises multiple polymerizable groups.

18. The stabilized polymersome made according to claim 1.

19. A stabilized polymersome, comprising:

a polymersome comprising a multiblock copolymer layer structure,

the layer structure comprising multiblock copolymer chains having hydrophilic and hydrophobic blocks, and

two or more chains being covalently bonded to one another.

20. The stabilized polymersome of claim 19, wherein the layer structure is characterized as being a bilayer.

21. The stabilized polymersome of claim 19, comprising two hydrophobic blocks covalently bonded to one another.

22. The stabilized polymersome of claim 19, wherein the stabilized polymersome is characterized as being biodegradable.

23. The stabilized polymersome of claim 19, wherein a hydrophilic block comprises poly(ethylene oxide), poly(acrylic acid), poly(ethylene glycol), or any combination thereof.

24. The stabilized polymersome of claim 19, wherein the hydrophobic block comprises poly(caprolactone), poly(methylcaprolactone), poly(menthide), poly(lactide), poly(glycolide), poly(methylglycolide), poly(dimethylsiloxane), poly(isobutylene), poly(styrene), poly(ethylene), poly(propylene oxide), or any combination thereof.

25. The stabilized polymersome of claim 19, wherein the stabilized polymersome further comprises one or more agents disposed within the layer structure, within the interior of the polymersome, on the surface of the polymersome, or any combination thereof.

26. The stabilized polymersome of claim 25, wherein an agent comprises a drug, a therapeutic compound, a nanoparticle, an imaging agent, a contrast agent, a nutrient, a vitamin, a protein, DNA, RNA, an oligonucleotide, a salt, a gene, a biological material, a magnetic material, a radioactive material, or any combination thereof.

27. The stabilized polymersome of claim 19, wherein the stabilized polymersome is capable of releasing an agent disposed within the polymersome, within the layer structure of the polymersome, or both, at a higher rate when the stabilized polymersome is exposed to a stimulus.

28. The stabilized polymersome of claim 27, wherein a stimulus comprises an acidic environment, an osmotic gradient, oxidative or reductive stress, or heat.

29. The stabilized polymersome of claim 27, further comprising one or more cross-links between one or more multiblock copolymer chains.

30. A method for delivering an agent to a subject, comprising:

introducing one or more stabilized polymersomes into a patient,

the one or more stabilized polymersomes having a layer structure comprising multiblock copolymer, the multiblock copolymer comprising hydrophobic and hydrophilic blocks, the one or more stabilized polymersomes comprising one or more therapeutic agents; and

exposing the one or more stabilized copolymers to a stimulus to effect release of the one or more therapeutic agents.

31. The method of claim 30, wherein the one or more stabilized polymersomes are taken up by one or more cells characterized by a diseased state.

32. The method of claim 30, wherein one or more cells in a diseased state expose the one or more stabilized polymersomes to the stimulus.

33. The method of claim 30, wherein the stabilized polymersome is used to treat a disease, to assist in imaging, or any combination thereof.

34. The method of claim 30, wherein exposing the stabilized polymersome to the stimulus effects an enhanced release of the one or more agents from the stabilized polymersome.

35. A method for altering the properties of a polymersome, comprising:

providing a polymersome,

the polymersome comprising a multiblock copolymer layer structure,

the structure comprising a plurality of multiblock copolymer chains,

the multiblock copolymer comprising hydrophilic and hydrophobic blocks; and

forming covalent bonds between two or more hydrophobic blocks of multiblock copolymer chains.

36. The method of claim 35, wherein the forming of covalent bonds is accomplished by polymerizing reactive groups present on the hydrophobic blocks.

37. The method of claim 35, further comprising forming one or more cross-links between copolymer chains.

38. The method of claim 35, wherein the layer structure comprises a bilayer.