SOYBEANS HAVING HIGH GERMINATION RATES AND ULTRA-LOW RAFFINOSE AND STACHYOSE CONTENT

• US 20110003045A1
• Filed: 04/16/2010
• Published: 01/06/2011
• Est. Priority Date: 04/16/2009
• Status: Active Grant

• Current Assignee: Benson Hill Seeds Incorporated (Benson Hill, Inc.), United States Department Of Agriculture
• Original Assignee: United States Department Of Agriculture
• Inventors: DIERKING, EMILY C., BILYEU, KRISTIN D., SCHILLINGER, JOHN A.
• Granted Patent: US 8,471,107 B2
• Time in Patent Office: Days
• US Class Current: 426/417
• CPC Class Codes: A01H 5/10 Seeds
• CPC Class Codes: C12N 15/8245 involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
• CPC Class Codes: C12N 9/1051 Hexosyltransferases (2.4.1)

Abstract

Soybean seeds having an ultra-low raffinose and stachyose phenotype. Also disclosed is a mutant allele of soybean designated SG-ULRFO which results in an ultra-low raffinose and stachyose phenotype. The present invention also relates to a soybean seed, a soybean plant and parts of a soybean plant and a soybean hybrid which comprises the mutant allele. Also disclosed are ultra-low raffinose and stachyose soybean seeds having unexpectedly increased germination rates when compared with soybean lines not having a low raffinose and stachyose seed content. Also disclosed are mutant RS3 and RS4 genes with polymorphisms which contribute to the ultra-low raffinose and stachyose phenotype as described in the present invention. The present invention also relates for method of using the soybean seeds and plants of the present invention, to plants parts derived from the present invention, to methods of producing transgenic plants using the plants and seeds of the present invention.

Claims

1. A soybean seed containing a mutant allele designated SG-ULRFO, wherein said allele confers an ultra-low raffinose and stachyose seed content and, wherein a representative sample of seed containing said SG-ULRFO mutant allele has been deposited with ATCC Accession No. PTA-______.

2. A soybean plant or a part thereof, produced by growing the seed of claim 1.

4. The soybean seed of claim 1, wherein the germination rate is at least 83%.

13. A method of producing a commodity plant product, comprising obtaining the plant of claim 2, or a part thereof, wherein the commodity plant product is protein concentrate, protein isolate, soybean hulls, meal, flour or oil and producing said commodity plant product therefrom.

3. A soybean seed having an ultra-low raffinose and stachyose seed content, wherein the raffinose seed content is less than 0.13% by dry or wet weight of the total seed content and wherein the stachyose seed content is less than 1.6% by dry or wet weight of the total seed content.

5. The soybean seed of claim 3, wherein the germination rate is at least 83%.

6. A soybean plant, or a part thereof, produced from growing the soybean seed of claim 3.

7. A soybean plant with a heritable phenotype of (i) an ultra-low raffinose seed content of less than 0.13% by dry or wet weight of the total seed content and (ii) an ultra-low stachyose seed content of less than 1.6% by dry or wet weight of the total seed content.

8. A nucleic acid sequence, wherein the nucleic acid sequence has an identity of at least 80% as set forth in SEQ ID NO:

3.

9. A nucleic acid sequence, wherein the nucleic acid sequence has an identity of at least 80% as set forth SEQ ID NO:

5.

10. A soybean seed comprising an amino acid sequence encoding an RS2 polypeptide having the amino acid sequence as set forth in SEQ ID NO:

2, an amino acid sequence encoding an RS3 polypeptide having the amino acid sequence as set forth in SEQ ID NO;

4 and an amino acid sequence encoding an RS4 polypeptide having the amino acid sequence as set forth in SEQ ID NO;

6.

11. A soybean plant, or a part thereof, produced by growing the seed of claim 10.

12. A vector comprising the nucleic acid sequence of SEQ ID NO:

1, the nucleic acid sequence of SEQ ID NO;

3 and the nucleic acid sequence of SEQ ID NO;

5.