Device and method for multiple analyte detection
First Claim
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1. A method comprising:
- pre-loading a first unlabeled oligonucleotide into a chamber;
pre-loading a second unlabeled oligonucleotide into the chamber;
after the pre-loading of the first unlabeled oligonucleotide and of the second unlabeled oligonucleotide, introducing a sample comprising a target sequence into the chamber;
reacting the target sequence with both the first unlabeled oligonucleotide and the second unlabeled oligonucleotide in the chamber, to form a reaction product in the chamber;
producing a detectable signal from the reaction product in the chamber; and
detecting more than one optical signal indicative of more than one different analyte in the sample, wherein the more than one optical signal comprises the detectable signal.
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Abstract
The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.
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15 Claims
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1. A method comprising:
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pre-loading a first unlabeled oligonucleotide into a chamber; pre-loading a second unlabeled oligonucleotide into the chamber; after the pre-loading of the first unlabeled oligonucleotide and of the second unlabeled oligonucleotide, introducing a sample comprising a target sequence into the chamber; reacting the target sequence with both the first unlabeled oligonucleotide and the second unlabeled oligonucleotide in the chamber, to form a reaction product in the chamber; producing a detectable signal from the reaction product in the chamber; and detecting more than one optical signal indicative of more than one different analyte in the sample, wherein the more than one optical signal comprises the detectable signal. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification