DETECTION OF NUCLEIC ACIDS BY TYPE-SPECIFIC HYBRID CAPTURE METHOD
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Accused Products
Abstract
Target-specific hybrid capture (TSHC) provides a nucleic acid detection method that is not only rapid and sensitive, but also highly specific and capable of discriminating highly homologous nucleic acid sequences. The method produces DNA/RNA hybrids which can be detected by a variety of methods.
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Citations
96 Claims
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1-24. -24. (canceled)
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25. A method of detecting a target nucleic acid consisting essentially of:
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a) hybridizing a single-stranded or partially single-stranded target nucleic acid to a capture sequence probe and a signal sequence probe, wherein the capture sequence probe and the signal sequence probe hybridize to non-overlapping regions within the target nucleic acid and not to each other, wherein said hybridization forms an RNA/DNA hybrid between said signal sequence probe and the target nucleic acid; and b) detecting the RNA/DNA hybrid by binding an antibody that recognizes the RNA/DNA hybrid to said hybrid, wherein said antibody is detectably labeled. - View Dependent Claims (26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 93, 96)
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43. A method of detecting a target nucleic acid consisting essentially of:
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a) hybridizing a single stranded or partially single-stranded target nucleic acid to a capture sequence probe and a signal sequence probe, wherein the capture sequence probe and the signal sequence probe hybridize to non-overlapping regions within the target nucleic acid and not to each other, wherein the signal sequence probe comprises a DNA/RNA hybrid region, wherein said hybridization forms a complex; and b) detecting said complex. - View Dependent Claims (44, 45, 46, 47, 48, 49, 50, 51, 52, 94)
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53. A nucleic acid probe consisting of a sequence selected from the group consisting of SEQ ID NO:
- 1 through SEQ ID NO;
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- 1 through SEQ ID NO;
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54-61. -61. (canceled)
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62. A method of detecting a target nucleic acid comprising:
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a) hybridizing a single-stranded or partially single-stranded target nucleic acid to a capture sequence probe and a signal sequence probe to form double-stranded hybrids between said probes and the target nucleic acid; b) binding the hybrid to a solid phase forming a bound hybrid; and c) detecting the bound hybrid, wherein the capture sequence probe and the signal sequence probe hybridize to non-overlapping regions within the target nucleic acid and not to each other, wherein step b) is carried out at about 20°
C. to about 90°
C.- View Dependent Claims (63, 64, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 83, 85, 86, 87, 88, 89, 90, 91, 92, 95)
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65-67. -67. (canceled)
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82. (canceled)
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84. (canceled)
Specification