MICROFLUIDIC DEVICE HAVING ONBOARD TISSUE OR CELL SAMPLE HANDLING CAPABILITY
First Claim
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1. A microfluidic device, comprising:
- a substrate;
a microfluidic flow channel formed in said substrate, wherein said flow channel extends through a portion of said substrate adapted to facilitate cytometry analysis of cells flowing in said flow channel; and
a sample repository onboard said substrate and containing material operative to preserve cells in a tissue sample placed within said sample repository.
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Abstract
The present disclosure is generally directed to systems for the storage and preservation of an original tissue or cell sample onboard a microfluidic device, such as a cytometry chip. In some embodiments, the sample may be disassociated while onboard the microfluidic device.
80 Citations
28 Claims
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1. A microfluidic device, comprising:
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a substrate; a microfluidic flow channel formed in said substrate, wherein said flow channel extends through a portion of said substrate adapted to facilitate cytometry analysis of cells flowing in said flow channel; and a sample repository onboard said substrate and containing material operative to preserve cells in a tissue sample placed within said sample repository. - View Dependent Claims (2, 3, 4, 5)
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6. A method for analyzing cells, comprising the steps of:
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a) providing a tissue sample; b) disassociating cells from said tissue sample; c) analyzing said disassociated cells by cytometry while said cells are onboard a microfluidic device having a substrate; and d) placing a non-disassociated portion of said tissue sample in a sample repository onboard said microfluidic device. - View Dependent Claims (7, 8, 9, 10, 11, 12, 13, 14)
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15. A microfluidic device, comprising:
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a substrate; a sample well onboard said substrate for holding a tissue sample; means for disassociating cells from said tissue sample while said tissue sample is in said sample well; and a microfluidic flow channel formed in said substrate and operatively coupled to said sample well for receiving said disassociated cells, wherein said flow channel extends through a portion of said substrate adapted to facilitate cytometry analysis of said cells flowing in said flow channel. - View Dependent Claims (16, 17, 18, 19)
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20. A method for analyzing cells, comprising the steps of:
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a) placing a tissue sample in a sample well onboard a microfluidic device; b) disassociating cells from said tissue sample within said sample well; and c) analyzing said disassociated cells by cytometry while said cells are onboard said microfluidic device. - View Dependent Claims (21, 22, 23, 24)
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25. A microfluidic device, comprising:
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a substrate; an input port operatively coupled to said substrate for accepting a quantity of cells; a microfluidic flow channel formed in said substrate, wherein said flow channel extends through a portion of said substrate adapted to facilitate cytometry analysis of said cells flowing in said flow channel; and a sample repository onboard said substrate and in fluid communication with said microfluidic flow channel; wherein a portion of said cells may be routed to said sample repository through said flow channel without undergoing cytometry analysis. - View Dependent Claims (26)
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27. A method for analyzing cells, comprising the steps of:
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a) providing a quantity of cells into a microfluidic flow channel formed in a substrate of a microfluidic device; b) depositing a first portion of said cells in a sample well onboard said substrate and in fluid communication with said microfluidic flow channel; and c) analyzing a second portion of said cells by cytometry while said cells are onboard a microfluidic device. - View Dependent Claims (28)
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Specification