MICROFLUIDIC DEVICE

• US 20110003325A1
• Filed: 07/06/2010
• Published: 01/06/2011
• Est. Priority Date: 07/06/2009
• Status: Active Grant

• Current Assignee: Sony Corporation (Sony Group Corp.), Sony Corporation Of America (Sony Group Corp.)
• Original Assignee: Sony Corporation (Sony Group Corp.), Sony Corporation Of America (Sony Group Corp.)
• Inventors: Durack, Gary P.
• Granted Patent: US 8,778,279 B2
• Time in Patent Office: Days
• US Class Current: 435/29
• CPC Class Codes: B01L 2200/0647 Handling flowable solids, e.g. microscopic beads, cells, particles
• CPC Class Codes: B01L 2200/147 Employing temperature sensors
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2400/0436 acoustic forces, e.g. surface acoustic waves [SAW]
• CPC Class Codes: B01L 2400/0475 specific mechanical means and fluid pressure
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: G01N 15/1484 microstructural devices
• CPC Class Codes: G01N 15/149 specially adapted for sorting particles, e.g. by their size or optical properties
• CPC Class Codes: G01N 2015/149 Sorting the particles
• CPC Class Codes: Y10T 436/25 including sample preparation

Abstract

The present disclosure relates to microfluidic devices adapted for facilitating cytometry analysis of particles flowing therethrough. In certain embodiments, the microfluidic devices have onboard sterilization capabilities. In other embodiments, microfluidic devices have integral collection bags and methods for keeping the microfluidic channels clean.

Claims

1. A microfluidic device, comprising:

a substrate;

a microfluidic flow channel formed in said substrate, wherein said flow channel extends through a portion of said substrate adapted to facilitate cytometry analysis of cells flowing in said flow channel;

a sterilization repository onboard said substrate, said sterilization repository being fluidically coupled to said flow channel; and

a substance disposed in said sterilization repository, said substance being operative to sterilize said flow channel when said substance flows from said sterilization repository into said flow channel.

2. The microfluidic device of claim 1, further comprising:

a valve disposed between said sterilization repository and said flow channel;

wherein fluid may flow from said sterilization repository to said flow channel when said valve is in an open position, and fluid is prevented from flowing from said sterilization repository to said flow channel when said valve is in a closed position.

3. The microfluidic device of claim 1, further comprising:

an input port fluidically coupled to said flow channel.

4. The microfluidic device of claim 3, further comprising:

a first sample well fluidically coupled to said flow channel;

a second sample well fluidically coupled to said flow channel; and

a flow diverter having a flow diverter input coupled to said flow channel, a first flow diverter outlet coupled to said first sample well, and a second flow diverter outlet coupled to said second sample well, said flow diverter having a first position and a second position;

wherein said flow diverter is operative to cause fluid in said flow channel to flow to said first sample well when said flow diverter is in a first position; and

wherein said flow diverter is operative to cause fluid in said flow channel to flow to said second sample well when said flow diverter is in a second position.

5. The microfluidic device of claim 4, wherein said flow diverter is selected from the group consisting of:

piezoelectric devices, air bubble insertion means, and magnetically actuated fluid deflectors.

6. The microfluidic device of claim 1, wherein a location of said sterilization repository is selected from the group consisting of:

on said substrate and in said substrate.

7. The microfluidic device of claim 1, wherein said material is selected from the group consisting of:

bleach and sodium hypochlorite.

8. The microfluidic device of claim 1, wherein said sterilization repository comprises a well formed in said substrate.

9. The microfluidic device of claim 8, further comprising:

a cover affixed to said substrate and substantially sealing said well.

10. A method of detecting particles in a sample, the method comprising the steps of:

a) providing a microfluidic device, said microfluidic device comprising;

a substrate;

a flow channel formed in said substrate for transport of a liquid sample;

a sterilization repository onboard said substrate, said sterilization repository being fluidically coupled to said flow channel;

a sterilizing substance disposed within said sterilization repository; and

a valve disposed between said sterilization repository and said flow channel;

b) flowing said sample through said flow channel; and

c) opening said valve such that said sterilizing substance contained in said sterilization repository may flow through said flow channel, thereby sterilizing said flow channel.

11. The method of claim 10, wherein said sterilizing substance is selected from the group consisting of:

bleach and sodium hypochlorite.

12. A microfluidic device, comprising:

a substrate;

a microfluidic flow channel formed in said substrate, wherein said flow channel extends through a portion of said substrate adapted to facilitate cytometry analysis of cells flowing in said flow channel, said flow channel having an outlet; and

a collection bag coupled to said substrate and having an inlet fluidically coupled to said flow channel outlet.

13. The microfluidic device of claim 12, wherein said collection bag comprises:

an inner bag fluidically coupled to said inlet; and

an outer bag enclosing said inner bag.

14. The microfluidic device of claim 12, wherein said collection bag comprises an artificial insemination straw.

15. A microfluidic device, comprising:

a substrate;

a microfluidic flow channel formed in said substrate, wherein said flow channel extends through a portion of said substrate adapted to facilitate cytometry analysis of cells flowing in said flow channel, said flow channel having an outlet;

a well onboard said substrate and fluidically coupled to said flow channel, said well having a porous surface; and

a collection bag coupled to said substrate and having an inlet fluidically coupled to said porous surface.

16. A method of detecting particles in a sample, the method comprising the steps of:

a) providing a microfluidic device, said microfluidic device comprising;

a substrate;

an input port;

a flow channel formed in said substrate for transport of a liquid sample, said flow channel fluidically coupled to said input port; and

an output port fluidically coupled to said flow channel;

b) flowing said sample through said flow channel;

c) attaching a collection bag to said output port;

d) allowing sample to flow from said flow channel and into said collection bag;

e) after step (d), detaching said collection bag from said output port; and

f) after step (e), attaching said collection bag to said input port.

17. A microfluidic device, comprising:

a substrate;

a microfluidic flow channel formed in said substrate, wherein said flow channel extends through a portion of said substrate adapted to facilitate cytometry analysis of cells flowing in said flow channel, said flow channel having an outlet;

a sheath fluid flow channel formed in said substrate and fluidically coupled to said microfluidic flow channel; and

a collection bag having a first chamber and a second chamber, said first chamber being fluidically coupled to said microfluidic flow channel outlet and said second chamber being fluidically coupled to said sheath fluid flow channel.

18. A microfluidic device, comprising:

a substrate;

a sheath fluid flow channel formed in said substrate, wherein said flow channel extends through a portion of said substrate adapted to facilitate cytometry analysis of cells flowing in said flow channel, said flow channel having an outlet;

a microfluidic flow channel formed in said substrate and having an outlet nozzle; and

wherein said microfluidic flow channel intersects said sheath fluid flow channel such that said outlet nozzle is positioned to inject said cells substantially in a center of said sheath fluid flow channel.

19. A method of detecting particles in a sample, the method comprising the steps of:

a) providing a microfluidic device, said microfluidic device comprising;

a substrate;

an input port;

a flow channel formed in said substrate for transport of a liquid sample, said flow channel fluidically coupled to said input port; and

an output port fluidically coupled to said flow channel;

b) mixing a detergent and a cell sample to create a mixture; and

c) flowing said mixture through said flow channel.