BIOMARKERS FOR THE PREDICTION OF RENAL INJURY
First Claim
Patent Images
1. A diagnostic marker selected from an isolated polynucleotide comprising the nucleic acid sequence set forth in any one of SEQ. ID NOs:
- 2, 4, 5, 7, 8, 10, 11, 13, 14, 16-19, 21-23, 25, 26, 28, 29, 31, 33, 35, 37, 39, 41, or a nucleic acid sequence having at least 90% identity to said sequence set forth in any one of SEQ ID NOs;
2, 4, 5, 7, 8, 10, 11, 13, 14, 16-19, 21-23, 25, 26, 28, 29, 31, 33, 35, 37, 39, 41, wherein the polynucleotide is present in at least one of a kidney tissue, body fluid or body secretion.
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Abstract
The present invention relates to means and methods for predicting the onset of renal injury based on measuring the expression of polynucleotides and proteins, particularly on measuring the expression of sets of novel as well as known polynucleotides and proteins, and to kits utilizing same.
15 Citations
32 Claims
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1. A diagnostic marker selected from an isolated polynucleotide comprising the nucleic acid sequence set forth in any one of SEQ. ID NOs:
- 2, 4, 5, 7, 8, 10, 11, 13, 14, 16-19, 21-23, 25, 26, 28, 29, 31, 33, 35, 37, 39, 41, or a nucleic acid sequence having at least 90% identity to said sequence set forth in any one of SEQ ID NOs;
2, 4, 5, 7, 8, 10, 11, 13, 14, 16-19, 21-23, 25, 26, 28, 29, 31, 33, 35, 37, 39, 41, wherein the polynucleotide is present in at least one of a kidney tissue, body fluid or body secretion. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
- 2, 4, 5, 7, 8, 10, 11, 13, 14, 16-19, 21-23, 25, 26, 28, 29, 31, 33, 35, 37, 39, 41, or a nucleic acid sequence having at least 90% identity to said sequence set forth in any one of SEQ ID NOs;
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9. A method for predicting onset of renal injury caused by treatment with a compound, comprising (a) administering a dose of the compound to at least one test subject;
- (b) after a selected time period, obtaining a biological sample from the at least one subject;
(c) measuring in the biological sample the expression level of at least two polynucleotide markers corresponding to sequences within at least two Candidates selected from ID NOs;
1, 5, 6, 12, 15 and 16; and
(d) determining whether the sample is in the positive class for onset of renal injury using a classifier comprising at least the two markers for which the expression level is measured. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
- (b) after a selected time period, obtaining a biological sample from the at least one subject;
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22. An isolated polynucleotide probe or primer comprising a nucleic acid sequence that specifically hybridizes to a marker having a nucleic acid sequence as set forth in any one of SEQ ID NOs:
- 1, 3, 6, 12, 48, 52, 2, 4, 5, 8, 13, 14, 49, 53, 254, 266, 329, 263, 287, 290, 296, and 332.
- View Dependent Claims (23)
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24. A kit for detecting renal injury, comprising at least two probes or pairs of primers and reagents for detecting at least two polynucleotide markers corresponding to sequences within any one of Candidates of ID NOs:
- 1, 5, 6, 12, 15 and 16.
- View Dependent Claims (25, 26, 27, 28, 29, 30, 31, 32)
Specification