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Compositions, devices, systems, and methods for using a nanopore

  • US 20110005918A1
  • Filed: 04/04/2008
  • Published: 01/13/2011
  • Est. Priority Date: 04/04/2007
  • Status: Abandoned Application
First Claim
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1. A method for controlling binding of an enzyme to a partially double-stranded polynucleotide complex, the method comprising:

  • providing two separate, adjacent pools of a medium and an interface between the two pools, the interface having a channel so dimensioned as to allow sequential monomer-by-monomer passage from one pool to the other pool of only one polynucleotide at a time;

    providing an enzyme having binding activity to a partially double-stranded polynucleotide complex;

    providing a polynucleotide complex comprising a first polynucleotide and a second polynucleotide, wherein a portion of the polynucleotide complex is double-stranded, and wherein the first polynucleotide further comprises a moiety that is incompatible with the second polynucleotide;

    introducing the polynucleotide complex into one of the two pools;

    introducing the enzyme into one of the pools;

    applying a potential difference between the two pools, thereby creating a first polarity;

    reversing the potential difference a first time, thereby creating a second polarity;

    reversing the potential difference a second time to create the first polarity, thereby controlling the binding of the enzyme to the partially double-stranded polynucleotide complex.

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