BIOSENSOR AND A RELATED MANUFACTURING METHOD

• US 20110008210A1
• Filed: 02/23/2009
• Published: 01/13/2011
• Est. Priority Date: 02/21/2008
• Status: Abandoned Application

• Current Assignee: Valtion Teknillinen Tutkimuskeskus
• Original Assignee: Valtion Teknillinen Tutkimuskeskus
• Inventors: Hast, Jukka, Kansakoski, Markku, Kopola, Harri, Kurkinen, Marika, Tuomikoski, Markus
• Application Number:
  US12/918,843
• Publication Number:
  US 20110008210A1
• Time in Patent Office: Days
• US Class Current: 422/52
• CPC Class Codes: G01N 2021/056 Laminated construction
• CPC Class Codes: G01N 21/648 using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
• CPC Class Codes: G01N 2201/0628 Organic LED [OLED]

Abstract

A biosensor platform for a biosensor adapted to detect one or more predetermined target analytes in a sample, includes a waveguide for transporting light emitted by a light source, at least one light source including an OLED, for incoupling light to the waveguide, the light source being arranged on the waveguide, a binding site including immobilized biorecognition material capable of binding to the target analytes, the binding site positioned relative to the waveguide such that evanescent field triggered by the light propagating in the waveguide extend to the binding site, a microfluidic layer including one or more microfluidic cavities for conveying the sample past the binding site to enable at least part of the target analytes of the sample to bind to the immobilized biorecognition material. The biosensor platform is configured to enable, when least part of the target analytes are bound to the immobilized biorecognition material, fluorescent markers associated with the bound target or other analytes to be excited by the evanescent field so as to emit fluorescence detectable by a detector.

Claims

1. A biosensor platform for a biosensor adapted to detect one or more predetermined target analytes in a sample, comprising

a waveguide (202) for transporting, optionally by substantially total internal reflection, light emitted by a light source,

at least one light source (204) including an organic light emitting diode (OLED) arranged on said waveguide for incoupling light to the waveguide, such as printed on the waveguide or provided with a layer on the waveguide,

a binding site (214) comprising immobilized biorecognition material capable of binding to the target analytes, said binding site positioned relative to the waveguide such that evanescent field triggered by the light propagating in the waveguide extends to the binding site, said binding site being optionally specifically arranged on a surface of the waveguide facing the microfluidic layer, and

a microfluidic layer (206) comprising one or more microfluidic cavities for conveying the sample past said binding site so as to enable at least part of the target analytes of the sample to bind to the immobilized biorecognition material,

wherein said biosensor platform is further configured to enable, when said at least part of the target analytes are bound to the immobilized biorecognition material, fluorescent markers associated with the bound target or other analytes to be excited by the evanescent field so as to emit fluorescence detectable by a detector.

2. The biosensor platform of claim 1, further comprising mobile biorecognition material provided with said fluorescent markers.

3. The biosensor platform of claim 2, wherein said mobile biorecognition material is configured to bind to the target analytes of the sample.

4. The biosensor platform of claim 1, further comprising one or more competitive analytes relative to the target analytes, the competitive analytes being provided with fluorescent markers and capable of binding to the immobilized biorecognition material.

5. The biosensor platform of claim 1, wherein said immobilized or mobile biorecognition material comprises antibody for a predetermined antigen, said antigen being defined as said target analyte.

6. The biosensor platform of claim 1, wherein said immobilized biorecognition material of the binding site comprises antigen capable of binding to predetermined antibody, or a group of antibodies, in the sample, said antibody or said group of antibodies of the sample being defined as said one or more target analytes.

7. The biosensor platform of claim 6, provided with additional antibody associated with said fluorescent markers and capable of binding to the antigen-antibody complexes formed on the binding site.

8. The biosensor platform of claim 1, wherein said microfluidic layer comprises at least one element selected from a group consisting of:

a source chamber for receiving the sample, a funneling means for conveying the sample deeper in the layer, a tube for transporting the sample, an intermediate chamber for gathering the sample, and a destination chamber for gathering the sample after biorecognition phase.

9. The biosensor platform of claim 1, configured to apply capillary action in conveying the sample.

10. The biosensor platform of claim 1, wherein the microfluidic layer and/or the waveguide include at least one polymeric material optionally selected from a group consisting of:

PET (polyethylene terephthalate), PEN (polyethylene naphthalate), PMMA (polymethyl methacrylate), PC (polycarbonate), and COC (cyclo-olefin copolymer).

11. The biosensor platform of claim 1, wherein layer thicknesses of the waveguide and/or the microfluidic layer are substantially equal or under about 500 μ

m.

12. The biosensor platform of claim 1, configured to detect a plurality of different target analytes.

13. A biosensor comprising the biosensor platform of claim 1 and a detector for measuring the emitted fluorescence.

14. The biosensor of claim 13, wherein said detector is external (218) to the platform.

15. The biosensor of claim 14, wherein said detector is a dedicated array detector, such as a CMOS- or CCD-based analysator, or a multi-purpose device, such as a consumer electronics apparatus provided with a camera.

16. The biosensor of claim 13, wherein said detector is integrated with the biosensor platform.

17. A method for manufacturing a biosensor platform as defined by claim 1, wherein at least one of said waveguide, microfluidic layer, light source, detector, binding site, and an aggregate entity comprising at least two of the aforesaid elements is produced utilizing a roll-to-roll technique.

18. The method of claim 17, wherein the light source is printed on the waveguide.

19. The method of claim 17, wherein at least part of said one or more microfluidic cavities are hot embossed to the microfluidic layer.

20. The method of claim 17, wherein said biorecognition material is spread to the binding site by dispensing, inkjetting, gravure printing, flexo printing, or screen printing.

21. The method of claim 17, wherein said biorecognition material is bound to the binding site by a non-covalent technique, passive adsorption, or by a covalent technique.