COMPARTMENTAL EXTRACT COMPOSITIONS FOR TISSUE ENGINEERING
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Abstract
The present invention concerns biologically-active cell-free scaffolds composed of extracts of cellular and/or extracellular compartments for use in tissue regeneration. The present invention also contemplates the novel concept of redesigning the biological scaffold by seeding cells thereon followed by cell elimination. Cells are seeded on the scaffold for a period of time during which a dynamic interaction occurs between the scaffold and the seeded cells, resulting in reshaping of the scaffold architecture and integration of newly synthesized matrix elements. Redesigning may improve the physical and biological characteristics of the scaffold, and also improve the matching of the scaffold to treat a specific target tissue or a specific patient, by seeding tissue-specific cells or by seeding cells which are autologous to a patient, respectively.
51 Citations
67 Claims
- 1. A scaffold composed of a cell extract.
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3-8. -8. (canceled)
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12. (canceled)
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19-36. -36. (canceled)
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37. A method of preparing a scaffold for use in conditions necessitating tissue or organ regeneration, repair or replacement comprising:
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(a) Obtaining cells and/or tissues; (b) Preparing a cell and/or a tissue extract; (c) Preparing a scaffold from said cell and/or tissue extract. - View Dependent Claims (39, 46, 47, 48, 49, 50, 51, 52, 53, 54)
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38. (canceled)
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40-45. -45. (canceled)
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55. (canceled)
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56. A method for producing a scaffold, comprising:
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(a) Obtaining cells or tissues; (b) Preparing extracellular extracts and/or intracellular extracts from said cells or tissues; (c) Preparing a scaffold from said extracellular and/or intracellular extracts; (d) Redesigning said scaffolds by seeding cells thereon; (e) Eliminating the cells from the scaffold; and
either(f) Administering the scaffold into a host in need thereof, or solubilizing the scaffold, thereby obtaining an injectable scaffold formulation. - View Dependent Claims (57, 58, 59, 60, 61)
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63-66. -66. (canceled)
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67. A cell culture device for culturing cells and producing extracellular matrix components, wherein said extracellular matrix components, cells and intracellular extracts produced there from, or any combination thereof are further used for fabricating a scaffold, and wherein said cell culture device is capable of exerting mechanical forces onto the cultured cells and is having a patterned surface.
Specification