MEASURING AMOUNT OF BOUND AND COMBINED NITRIC OXIDE IN BLOOD
First Claim
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1. A method for liberating nitric oxide gas from combined nitric oxide in a blood sample, comprising directing low power electromagnetic radiation at the blood sample for a period sufficient to release free nitric oxide from the combined nitric oxide.
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Abstract
Amount of combined nitric oxide or nitric oxide present as iron nitrosyls in a blood sample is determined by directing a low power electromagnetic radiation beam at a blood sample to liberate nitric oxide gas, dissolving the liberated nitric oxide gas and electrochemically detecting amount of dissolved nitric oxide gas.
45 Citations
27 Claims
- 1. A method for liberating nitric oxide gas from combined nitric oxide in a blood sample, comprising directing low power electromagnetic radiation at the blood sample for a period sufficient to release free nitric oxide from the combined nitric oxide.
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10. A method for determining amount of combined nitric oxide in a blood sample, comprising the steps of:
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(a) introducing a sample of the blood to be analyzed for amount of combined nitric oxide therein, into a sample containing zone having a front side which is electromagnetic radiation transparent and a rear side which is porous to the extent of permitting nitric oxide gas to pass therethrough while preventing protein from passing therethrough; (b) directing low power electromagnetic radiation at said front side to cause liberation of nitric oxide gas from combined nitric oxide and passage of the liberated nitric oxide gas from said rear side; (c) providing a solvent containing zone to dissolve the liberated nitric oxide gas that has passed through said rear side where the solvent is one that dissolves nitric oxide gas; (d) electrochemically detecting amount of dissolved nitric oxide gas in the solvent which corresponds to the total amount of nitric oxide present as combined nitric oxide in the sample. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18)
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19. A method for determining amount of nitric oxide present as combined nitric oxide and also amount of nitric oxide present as iron nitrosyls in blood comprising:
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(a) obtaining two samples of blood from the same source, each comprising combined nitric oxide present as nitrosothiols and iron nitrosyls and where one of the samples is denoted as the first sample and the other of the samples is denoted as the second sample; (b) treating the second sample with a nitrosothiols degrading agent to cause decomposition of nitrosothiols to nitrous acid and leaving iron nitrosyls in the sample; (c) analyzing for amount of nitric oxide present as combined nitric oxide in the first sample by steps comprising (i) introducing the first sample into a first sample containing zone which has a front side which is electromagnetic radiation transparent and a rear side which is porous to the extent of permitting nitric oxide gas to pass therethrough while preventing protein from passing therethrough, (ii) directing low power electromagnetic radiation at said front side of the first sample containing zone to cause liberation of nitric oxide gas from combined nitric oxide and passage of the liberated nitric oxide gas through said rear side, (iii) providing a first solvent containing zone to dissolve the liberated nitric oxide gas that has passed through said rear side where the solvent is one that dissolves nitric oxide gas, (iv) electrochemically detecting amount of dissolved nitric oxide in the first solvent containing zone which corresponds to the total amount of nitric oxide present as combined nitric oxide in said first sample, (d) analyzing for amount of nitric oxide present in the iron nitrosyls left in the step (b) treated second sample by steps comprising (i) introducing the treated second sample into a second sample containing zone which has a front side which is electromagnetic radiation transparent and a rear side which is porous to the extent of permitting nitric oxide gas to pass therethrough while preventing protein from passing therethrough, (ii) directing low power electromagnetic radiation at said front side of the second sample containing zone to cause liberation of nitric oxide gas from said iron nitrosyls and passing of the liberated nitric oxide gas through said rear side, (iii) providing a second solvent containing zone to dissolve the liberated nitric oxide gas that has passed through said rear side where the solvent is one that dissolves nitric oxide, (iv) electrochemically detecting amount of dissolved nitric oxide in the second solvent containing zone which corresponds to the amount of nitric oxide present as iron nitrosyls in said second sample. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27)
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Specification