METHOD OF DETECTING BIOPRODUCTS USING LOCALIZED SURFACE PLASMON RESONANCE SENSOR OF GOLD NANOPARTICLES
First Claim
1. A method of detecting bioproducts characterized by comprising the steps of:
- (a) immobilizing receptors onto a localized surface plasmon resonance (LSPR) sensor comprised of gold nanoparticles, the surface of which is modified with an organic adsorbent, and a cover glass where the gold nanoparticles are fixed;
(b) flowing a target material on the sensor having the immobilized receptors and(c) determining light-scattering spectra by using dark-field microscopy and a resonant Rayleigh scattering micro-spectroscopy system and analyzing mobility of the maximum wavelength.
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Abstract
Disclosed is a method of detecting bioproducts using Localized Surface Plasmon Resonance (LSPR) of gold nanoparticles, which can diagnose bioproducts based on changes in the maximum wavelength occurred by an antigen-antibody reaction after immobilization of the gold nanoparticles onto a glass panel. A sensor using such method exhibits high sensitivity, is low in price, and makes quick diagnosis possible, thereby being applicable to various biological fields associated with environmental contaminants, pathogens and the like, as well as diagnosis of diseases. Further, it provides a technology for manufacturing a sensor having higher sensitivity, low price and quick performance, as compared to conventional methods using SPR.
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Citations
13 Claims
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1. A method of detecting bioproducts characterized by comprising the steps of:
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(a) immobilizing receptors onto a localized surface plasmon resonance (LSPR) sensor comprised of gold nanoparticles, the surface of which is modified with an organic adsorbent, and a cover glass where the gold nanoparticles are fixed; (b) flowing a target material on the sensor having the immobilized receptors and (c) determining light-scattering spectra by using dark-field microscopy and a resonant Rayleigh scattering micro-spectroscopy system and analyzing mobility of the maximum wavelength. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A method of detecting PSA, characterized by comprising the steps of:
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(a) immobilizing PSA onto a localized surface plasmon resonance (LSPR) sensor comprised of gold nanoparticles, the surface of which is modified with an organic adsorbent, and a cover glass where the gold nanoparticles are immobilized (b) flowing PSA-ACT complex onto the sensor having the immobilized PSA and (c) determining light-scattering spectra by using dark-field microscopy and a resonant Rayleigh scattering micro-spectroscopy system and analyzing mobility of the maximum wavelength.
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10. A method of manufacturing a localized surface plasmon resonance (LSPR) sensor for detecting bioproducts, characterized by comprising the steps of:
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(a) preparing Au nanoparticles; (b) dispersing and immobilizing the Au nanoparticles onto a cover glass; and (c) conducting surface treatment of the immobilized Au nanoparticles with an organic adsorbent. - View Dependent Claims (11, 12, 13)
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Specification