METHOD FOR PREPARING GYNOSTEMMA PENTAPHYLLUM EXTRACT WITH INCREASING DAMULIN A AND DAMULIN B CONTENTS, AND PHARMACEUTICAL COMPOSITIONS OF THE SAME FOR TREATING METABOLIC DISEASE
First Claim
1. A method for preparing a novel Gynostemma pentaphyllum extract having increased concentrations of damulin A and damulin B, comprising the step of:
- treating a concentrate of an ethanol extract of Gynostemma pentaphyllum leaves with a high temperature and/or high pressure reaction for 0.1˜
24 hours, wherein the temperature is about 40-125°
C. and the pressure is about 1.2˜
1100 atmospheres and wherein the increased concentrations of damulin A and damulin B are 0.7-7% (w/w) and 0.5-6% (w/w), respectively, based on the total weight of a dry powder of the novel Gynostemma pentaphyllum extract.
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Abstract
Disclosed is an AMPK activating material used for improving and treating metabolic syndrome, in which AMPK (AMP-activated protein kinase) is a main enzyme for regulating an energy sensor and lipid/glucose metabolism in the body. The activation of AMPK inhibits the synthesis of fat and cholesterol, and accelerates the reduction of body fat and blood glucose, thereby improving obesity, diabetes, and hyperlipidaemia. The disclosed AMPK activating material contains, as active ingredients having an improving and treating effect on metabolic syndrome, including obesity, diabetes, and hyperlipidaemia, a novel compound 2α,3β,12β-trihydroxydammar-20(22)-E,24-diene-3-O-[β-D-glucopyranosyl-(1→)-β-D-glucopyranoside], named Damulin A, and a novel compound 2α,3β,12β-trihydroxydammara-20,24-diene-3-O-[β-D-glucopyranosyl-(1→)-β-D-glucopyranoside], named Damulin B. Herein, the contents of damulin A and damulin B (as active indicator ingredients for AMPK activation) can be increased by treating a Gynostemma pentaphyllum extract with high temperature/high pressure. Accordingly, the novel Gynostemma pentaphyllum extract with a significantly increased AMPK activating capability can be used for improving or treating metabolic syndrome, such as obesity, diabetes, and hyperlipidaemia.
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Citations
16 Claims
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1. A method for preparing a novel Gynostemma pentaphyllum extract having increased concentrations of damulin A and damulin B, comprising the step of:
treating a concentrate of an ethanol extract of Gynostemma pentaphyllum leaves with a high temperature and/or high pressure reaction for 0.1˜
24 hours, wherein the temperature is about 40-125°
C. and the pressure is about 1.2˜
1100 atmospheres and wherein the increased concentrations of damulin A and damulin B are 0.7-7% (w/w) and 0.5-6% (w/w), respectively, based on the total weight of a dry powder of the novel Gynostemma pentaphyllum extract.- View Dependent Claims (2, 3, 4, 5, 6, 7, 11)
- 8. A pharmaceutical composition for improving and treating a metabolic syndrome, comprising, as an active ingredient, at least one of damulin A and damulin B, represented by Formula 1:
- 10. A novel Gynostemma pentaphyllum extract comprising 0.7-7% (w/w) of damulin A and 0.5-6% (w/w) of damulin B based on the total weight of a dry powder of the Gynostemma pentaphyllum extract.
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14. Damulin A represented by the following formula:
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15. Damulin B represented by the following formula:
Specification