Systems and Methods for Multiple Analyte Detection

• US 20110020179A1
• Filed: 05/27/2010
• Published: 01/27/2011
• Est. Priority Date: 04/26/2005
• Status: Active Grant

• Current Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Ermakov, Sergey V., Liu, David M., Chiang, Yuh-Min, Van Camp, John, Dromaretsky, Alexander, Beard, Nigel P., Lehto, Dennis, Oldham, Mark F., Harding, Ian A., Shariati, Maryam, Ulmanella, Umberto, Roy, Joy, Yue, Min, Vann, Charles S., Yang, Joon-Mo
• Granted Patent: US 8,597,590 B2
• Time in Patent Office: Days
• US Class Current: 422/63
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01L 2200/0684 Venting, avoiding backpressure, avoid gas bubbles
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/041 Connecting closures to device or container
• CPC Class Codes: B01L 2300/048 enabling gas exchange, e.g. vents
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/0655 pinch valves
• CPC Class Codes: B01L 2400/0694 vents used to stop and induce flow, backpressure valves
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502723 characterised by venting arrangements
• CPC Class Codes: B01L 3/50853 with covers or lids
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: G01N 35/028 : having reaction cells in the form of microtitration plates
• CPC Class Codes: Y10T 436/2575 : Volumetric liquid transfer

Abstract

Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials.

Claims

1. A system for distribution of a biological sample, the system comprising:

a substrate, wherein the substrate comprises a main fluid supply channel connected to and fluidly communicating with a plurality of sample introduction channels, each sample introduction channel being connected to and fluidly communicating with a sample chamber, each sample chamber being connected to and fluidly communicating with a venting channel;

a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate; and

a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber,

wherein the substrate is constructed of detection-compatible and assay-compatible materials.

2. The system of claim 1, wherein the sealing instrument is configured to be placed in contact with an exterior portion of the substrate.

3. The system of claim 1, wherein the sealing instrument is configured to be placed in at least one of pressure contact and thermal contact with the substrate.

4. The system of claim 1, wherein the sealing instrument is configured to seal the sample introduction channels and venting channels for each sample chamber.

5. The system of claim 1, wherein the sealing instrument is configured to be placed in contact with the substrate at least one of before a reaction process that occurs in the sample chambers and during a reaction process that occurs in the sample chambers.

6. The system of claim 5, wherein the sealing plate is configured to be placed in contact with the substrate before a reaction process that occurs in the sample chambers to seal each sample chamber.

21. The system of claim 1, wherein the substrate further comprises a film layer and a base, and at least one venting mechanism configured to permit gas to escape the substrate.

7-20. -20. (canceled)

22-57. -57. (canceled)

58. A system for distribution of a biological sample, the system comprising:

means for distributing the sample to a plurality of sample chambers containing a preloaded reagent, the means for distributing comprising a main fluid supply channel connected to and fluidly communicating with a plurality of sample introduction channels, each sample introduction channel being connected to and fluidly communicating with a respective one of the plurality of sample chambers;

means for venting each of the sample chambers; and

means for sealing each of the sample chambers.

59. A device for testing a biological sample, the device comprising:

a substrate defining a plurality of distribution portions configured to distribute biological sample throughout the substrate, the plurality of distribution portions comprising a main fluid supply channel connected to and fluidly communicating with a plurality of sample introduction channels, each sample introduction channel being connected to and fluidly communicating with a sample chamber, each sample chamber being connected to and fluidly communicating with a venting channel; and

a substance disposed in at least one of the distribution portions, the substance being configured to seal each sample chamber so as to substantially prevent sample disposed in each sample chamber from flowing out of each sample chamber during testing of the biological sample.

60. The device of claim 59, wherein the substance comprises one of an adhesive, a gas, a substance configured to dissolve into a gas upon contact with the biological sample, a fluid immiscible with the biological sample, an absorbent material, and porous hydrophobic material.

61. The device of claim 59, wherein the substance is disposed in the sample introduction and venting channels for each sample chamber.

62. The device of claim 59, wherein the plurality of distribution portions further comprises a vent chamber in flow communication with each venting channel and wherein the substance is disposed in each vent chamber.

63. The device of claim 59, wherein the substance is disposed in the main fluid supply channel.

64. The device of claim 59, wherein the substance is configured to seal each sample chamber upon sample contacting the substance.

65. The device of claim 59, wherein the substance is configured to seal each sample chamber in response to elevating a temperature of the substrate during testing of the biological sample.