Microfluidic method and system for isolating particles from biological fluid
First Claim
1. A method for isolating particles from biological fluid of a patient, comprising the steps of:
- obtaining a sample of biological fluid directly from a cannula coupled to the patient, wherein the sample contains particles of multiple sample particle types, each sample particle type being classified as a targeted particle or a non-targeted particle;
tagging particles in the sample with tagging agents, including mixing the sample with a solution of magnetic tagging agents that selectively bind to particles of at least one of the sample particle types, thereby forming a group of tagged particles and another group of untagged particles within the sample;
passing the sample through a magnetic conduit having a magnetic field that interacts with at least a portion of the tagged particles; and
sorting the particles of the sample into at least two groups based on the interaction between the tagged particles and the magnetic field.
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Abstract
The invention relates to a system and method for isolating particles from a biological fluid, including: obtaining a sample of biological fluid from a source (e.g. a patient) in which the sample contains particles of multiple sample particle types; tagging particles in the sample with tagging agents, including mixing the sample with a solution of magnetic tagging agents that selectively bind to particles of at least one of the sample particle types, thereby forming a group of tagged particles in the sample; passing the sample through a magnetic conduit having a magnetic field that interacts with at least some of the tagged particles; sorting the particles of the sample into multiple groups based on the interaction between the tagged particles and the magnetic field; and optionally returning selected portions of the processed fluid back to its source with or without the addition of appropriate therapeutic agents.
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Citations
40 Claims
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1. A method for isolating particles from biological fluid of a patient, comprising the steps of:
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obtaining a sample of biological fluid directly from a cannula coupled to the patient, wherein the sample contains particles of multiple sample particle types, each sample particle type being classified as a targeted particle or a non-targeted particle; tagging particles in the sample with tagging agents, including mixing the sample with a solution of magnetic tagging agents that selectively bind to particles of at least one of the sample particle types, thereby forming a group of tagged particles and another group of untagged particles within the sample; passing the sample through a magnetic conduit having a magnetic field that interacts with at least a portion of the tagged particles; and sorting the particles of the sample into at least two groups based on the interaction between the tagged particles and the magnetic field. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
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33. A microfluidic system for isolating particles from biological fluid of a patient, comprising:
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a sampling module including a cannula coupled to the patient that obtains a sample of biological fluid from the patient, wherein the sample contains particles of multiple sample particle types; a microfluidic tagging conduit, fluidly coupled to the sampling module at a point-of-care of the patient, having; a first inlet that receives the sample; a second inlet that receives a solution of magnetic tagging agents that selectively bind to and tag particles of at least one particle type; and a textured surface that induces mixing of the particles and tagging agents; and a microfluidic magnetic conduit, fluidly coupled to the tagging conduit and having at least one magnet providing a magnetic field that interacts with at least a portion of the tagged particles such that the particles in the sample are sorted into at least two groups based on the interaction between the tagged particles and the magnetic field. - View Dependent Claims (34, 35, 36, 37, 38, 39, 40)
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Specification