RED FLUORESCENT PROTEINS WITH ENHANCED BACTERIAL EXPRESSION, INCREASED BRIGHTNESS AND REDUCED AGGREGATION
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Abstract
Polynucleotides encoding variant polypeptides of DsRed are provided herein. The DsRed variants have increased bacterial expression, reduced aggregation, increased solubility, shifted emission spectra or increased brightness relative to a wild-type DsRed.
77 Citations
62 Claims
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- 7. An isolated polynucleotide encoding a variant polypeptide of DsRed, the variant polypeptide having reduced aggregation relative to wild-type DsRed, the variant polypeptide comprising at least one amino acid substitution selected from the group consisting of E10P and Q188K.
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Specification