AGENTS FOR STIMULATING ACTIVITY OF METHYL MODIFYING ENZYMES AND METHODS OF USE THEREOF
First Claim
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1. A method of evaluating a test compound, the method comprising:
- contacting a histone methyl modifying enzyme and a substrate with a test compound in the presence of a stimulating agent;
evaluating activity of the histone methyl modifying enzyme on the substrate in the presence of the test compound, relative to a control, wherein a change in activity of the histone methyl modifying enzyme in the presence of the test compound, relative to the control, indicates that the test compound is a modulator of the histone methyl modifying enzyme.
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Abstract
Agents for stimulating activity of methyl modifying enzymes and methods of using the enzymes in assays to identify enzyme modulators are provided herein.
95 Citations
77 Claims
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1. A method of evaluating a test compound, the method comprising:
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contacting a histone methyl modifying enzyme and a substrate with a test compound in the presence of a stimulating agent; evaluating activity of the histone methyl modifying enzyme on the substrate in the presence of the test compound, relative to a control, wherein a change in activity of the histone methyl modifying enzyme in the presence of the test compound, relative to the control, indicates that the test compound is a modulator of the histone methyl modifying enzyme. - View Dependent Claims (3, 4, 5, 10, 11, 12, 13, 14, 15, 18, 24, 25, 26, 27, 28, 29, 30, 31, 32, 35, 36, 39, 74, 75, 76, 77)
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2. (canceled)
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6-9. -9. (canceled)
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16-17. -17. (canceled)
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19-23. -23. (canceled)
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33-34. -34. (canceled)
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37-38. -38. (canceled)
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40. A reaction mixture comprising:
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a histone methyl modifying enzyme; a substrate; and a stimulating agent, wherein the stimulating agent is present in an amount sufficient to increase activity of the histone methyl modifying enzyme.
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41-73. -73. (canceled)
Specification