SILKWORM GENOME EDITING WITH ZINC FINGER NUCLEASES
First Claim
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1. A genetically modified silkworm comprising at least one edited chromosomal sequence.
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Abstract
The present invention provides a genetically modified silkworm or cell comprising at least one edited chromosomal sequence. In particular, the chromosomal sequence is edited using a zinc finger nuclease-mediated editing process. The disclosure also provides zinc finger nucleases that target specific chromosomal sequences in the silkworm genome.
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23 Claims
- 1. A genetically modified silkworm comprising at least one edited chromosomal sequence.
- 20. A silkworm egg embryo, the embryo comprising at least one RNA molecule encoding a zinc finger nuclease that recognizes a chromosomal sequence and is able to cleave a site in the chromosomal sequence, and, optionally, (i) at least one donor polynucleotide comprising a sequence that is flanked by an upstream sequence and a downstream sequence, the upstream and downstream sequences having substantial sequence identity with either side of the site of cleavage or (ii) at least one exchange polynucleotide comprising a sequence that is substantially identical to a portion of the chromosomal sequence at the site of cleavage and which further comprises at least one nucleotide change.
- 22. A genetically modified silkworm cell comprising at least one edited chromosomal sequence.
Specification