FELINE GENOME EDITING WITH ZINC FINGER NUCLEASES
First Claim
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1. A genetically modified feline comprising at least one edited chromosomal sequence.
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Abstract
The present invention provides a genetically modified feline or cell comprising at least one edited chromosomal sequence. In particular, the chromosomal sequence is edited using a zinc finger nuclease-mediated editing process. The disclosure also provides zinc finger nucleases that target specific chromosomal sequences in the feline genome.
123 Citations
33 Claims
- 1. A genetically modified feline comprising at least one edited chromosomal sequence.
- 18. A feline embryo, the embryo comprising at least one RNA molecule encoding a zinc finger nuclease that is able to cleave a site in the chromosomal sequence, and, optionally, (i) at least one donor polynucleotide comprising a sequence that is flanked by an upstream sequence and a downstream sequence, the upstream and downstream sequences having substantial sequence identity with either side of the site of cleavage or (ii) at least one exchange polynucleotide comprising a sequence that is substantially identical to a portion of the chromosomal sequence at the site of cleavage and which further comprises at least one nucleotide change.
- 20. A genetically modified feline cell comprising at least one edited chromosomal sequence.
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26. A zinc finger nuclease, the zinc finger nuclease comprising a zinc finger DNA binding domain and a cleavage domain, the zinc finger nuclease having an amino acid sequence that has at least about 80% sequence identity to a sequence chosen from SEQ ID NOs:
- 1, 2, 3, 4, 5, 6, 7, and 8, or the zinc finger DNA binding domain helices have an amino acid sequence that is at least about 80% identical to a sequence chosen from SEQ ID NOs;
19, 20, 21, 22, 23, 24, 25, and 26. - View Dependent Claims (27, 28, 29, 31)
- 1, 2, 3, 4, 5, 6, 7, and 8, or the zinc finger DNA binding domain helices have an amino acid sequence that is at least about 80% identical to a sequence chosen from SEQ ID NOs;
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30. The zinc finger nuclease of claim 36, wherein the sequence identity is at least about 85%, 90%, 95%, or 100%.
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32. A nucleic acid sequence bound by a zinc finger nuclease, the nucleic acid sequence having at least 80% sequence identity with a sequence chosen from SEQ ID NOs:
- 9, 10, 11, 12, 13, 14, 15, 16, 27, 28, 29, 30, 31, 32, 33, and 34.
- View Dependent Claims (33)
Specification