Targeted integration into stem cells
First Claim
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1. A stem cell comprising a transgene, wherein the transgene comprises a gene product, wherein the transgene is integrated into a specified region of the genome of the stem, cell such that gene product is transcribed or translated upon or during differentiation of the stem cell into a lineage-specific or mature cell type.
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Abstract
Disclosed herein are methods and compositions for targeted integration of sequences of interest such as lineage-specific or cell fate reporter constructs or protein encoding sequences into stem cells.
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Citations
19 Claims
- 1. A stem cell comprising a transgene, wherein the transgene comprises a gene product, wherein the transgene is integrated into a specified region of the genome of the stem, cell such that gene product is transcribed or translated upon or during differentiation of the stem cell into a lineage-specific or mature cell type.
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19. A method of reducing or eliminating positional variation effects associated with transgene insertion into a specified region of a stem cell, the method comprising:
providing a stem cell in which the transgene inserted at one allele of the specified region comprises a gene expression construct comprising a regulatable construct and in which the other allele of the specified region comprises a transactivator for the regulatable promoter.
Specification