ASSAY FOR MYCOBACTERIUM AVIUM/INTRACELLULARE NUCLEIC ACID
First Claim
1. A method for detecting whether a sample contains nucleic acid from Mycobacterium avium (M. avium) or Mycobacterium intracellulare (M. intracellulare), said method comprising:
- assessing the presence or absence of a first target nucleic acid sequence in said sample; and
assessing the presence or absence of a second target nucleic acid sequence in said sample;
identifying the sample as containing nucleic acid from M. avium, M. intracellulare, or M. avium serovars 2/3, wherein the presence of said first target nucleic acid indicates that the sample contains M. avium, wherein the absence of said first target nucleic acid and the presence of said second target nucleic acid indicates that the sample contains M. intracellulare, and wherein the presence of said first and second target nucleic acids indicates that the sample contains M. avium serovar 2 or M. avium serovar 3,wherein said first target nucleic acid sequence is specific for the macrophage-induced gene (mig) gene of M. avium and said second target nucleic acid sequences is specific for the insertion sequence transposase (IS1245) gene of M. intracellulare, M. avium serovar 2 and M. avium serovar 3 andwherein said sample is not a gastric aspirate or a stool sample.
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Abstract
Disclosed is a method for determining the presence of Mycobacterium avium complex nucleic acids in a biological sample. In particular, the mig gene of M. avium and the DT1 gene of M. intracellulare are detected, preferably following amplification. In addition, the method distinguishes between species of M. avium and M. intracellulare. Also described are oligonucleotides that can be used as primers to amplify target genes such as mig and DT1 genes and as probes as well as kits containing the oligonucleotides.
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Citations
22 Claims
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1. A method for detecting whether a sample contains nucleic acid from Mycobacterium avium (M. avium) or Mycobacterium intracellulare (M. intracellulare), said method comprising:
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assessing the presence or absence of a first target nucleic acid sequence in said sample; and assessing the presence or absence of a second target nucleic acid sequence in said sample; identifying the sample as containing nucleic acid from M. avium, M. intracellulare, or M. avium serovars 2/3, wherein the presence of said first target nucleic acid indicates that the sample contains M. avium, wherein the absence of said first target nucleic acid and the presence of said second target nucleic acid indicates that the sample contains M. intracellulare, and wherein the presence of said first and second target nucleic acids indicates that the sample contains M. avium serovar 2 or M. avium serovar 3, wherein said first target nucleic acid sequence is specific for the macrophage-induced gene (mig) gene of M. avium and said second target nucleic acid sequences is specific for the insertion sequence transposase (IS1245) gene of M. intracellulare, M. avium serovar 2 and M. avium serovar 3 and wherein said sample is not a gastric aspirate or a stool sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method for diagnosing a subject infected with Mycobacterium avium (M. avium) or Mycobacterium intracellulare (M. intracellulare), comprising;
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contacting a biological sample containing nucleic acids from the subject with a first oligonucleotide specific for a first target nucleic acid sequence and a second oligonucleotide specific for a second target nucleic acid sequence; and determining if said first or second oligonucleotide has hybridized to said sample nucleic acids, diagnosing the subject as infected with M. avium, M. intracellular, or M. avium serovar 2/3 wherein hybridization of the first oligonucleotide is indicative of infection by M. avium, hybridization of the second oligonucleotide but not the first oligonucleotide is indicative of infection by M. intracellulare, and hybridization of the first and second oligonucleotides is indicative of infection by M. avium serovar 2 or M. avium serovar 3; and wherein said first target nucleic acid sequence is specific for the macrophage-induced gene (mig) gene of M. avium and said second target nucleic acid sequences is specific for the IS1245 gene of M. intracellulare, M. avium serovar 2 and M. avium serovar 3 and wherein said sample is not a gastric aspirate or stool sample. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22)
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Specification