METHOD OF DYNAMICALLY CULTURING EMBRYONIC STEM CELLS

• US 20110027887A1
• Filed: 10/18/2010
• Published: 02/03/2011
• Est. Priority Date: 11/29/2002
• Status: Abandoned Application

• Current Assignee: TECHNION RESEARCH AND DEVELOPMENT FOUNDATION, LTD. (Technion-Israel Institute Of Technology)
• Original Assignee: TECHNION RESEARCH AND DEVELOPMENT FOUNDATION, LTD. (Technion-Israel Institute Of Technology)
• Inventors: GERECHT-NIR, SHARON, ITSKOVITZ-ELDOR, JOSEPH
• Application Number:
  US12/906,155
• Publication Number:
  US 20110027887A1
• Time in Patent Office: Days
• US Class Current: 435/394
• CPC Class Codes: C12N 5/00 Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor; plant reproduction by tissue culture techniques A01H4/00
• CPC Class Codes: C12N 5/0603 Embryonic cells production of embryos, nuclear transfer A01K67/027; Embryoid bodies

Abstract

The present invention is of a method of dynamically generating human embryoid bodies which can be used for generating lineage specific cells and cell lines. Specifically, the present invention can be used to generate ESC-differentiated cells for cell-replacement therapy.

Claims

1. A method of dynamically generating embryoid bodies comprising culturing embryonic stem cells in a bioreactor under a randomized microgravitational force of 0.05-0.9 g without formation of gas bubbles to thereby obtain dynamically generated embryoid bodies.

2. The method of claim 1, wherein said microgravitational force is 0.1 G.

3. The method of claim 1, wherein said bioreactor is capable of maintaining said embryonic stem cells in a constant state of free fall during said culturing.

4. The method of claim 1, wherein said bioreactor is a tubular vessel having at least one vessel port for transferring materials into and out of said tubular vessel.

5. The method of claim 4, wherein said tubular vessel is rotating around a horizontal longitudinal axis.

6. The method of claim 1, wherein said bioreactor is capable of holding a fluid volume selected of up to 500 ml.

7. The method of claim 4, wherein a diameter of said tubular vessel is selected from range of 2.54-15.24 centimeters.

8. The method of claim 4, wherein said culturing of said embryonic stem cells is effected in said tubular vessel under a rotational speed selected from a range of 15-20 rpm.

9. The method of claim 1, wherein said bioreactor enables oxygenation of said fluid via controlled diffusion of dissolved gasses.