DEVICE FOR MICROSCOPY HAVING SELECTIVE ILLUMINATION OF A PLANE
First Claim
1. A microscopy device comprising:
- illumination means for generating an illumination beam, an imaging detector for spatially resolved acquisition of an emission radiation emitted by an object to be examined;
an illumination beam path between the illumination means and the object to be examined, and a detection beam path between the object to be examined and the detector,wherein the illumination beam path comprises illumination optics which are designed to generate a light sheet of illumination radiation extending transverse to the axis of the illumination beam path,wherein the axis of the detection beam path is oriented at an angle diverging from 0°
to a section plane of the light sheet and of the object to be examined, andwherein the illumination means comprise a pulsed laser.
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Accused Products
Abstract
A microscopy device, particularly for use in an imaging fluorescence lifetime microscopy method is provided. The microscopy device comprises an illumination means for generating an illumination beam, an imaging detector for spatially resolved acquisition of an emission radiation emitted by an object to be examined, an illumination beam path between the illumination means and the object to be examined, and a detection beam path between the object to be examined and the detector. The illumination beam path comprises illumination optics which are designed to generate a light sheet of illumination radiation extending transverse to the axis of the illumination beam path, wherein the axis of the detection beam path is oriented substantially perpendicular to a section plane of the light sheet and of the object to be examined. The illumination means comprise a pulsed laser.
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Citations
19 Claims
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1. A microscopy device comprising:
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illumination means for generating an illumination beam, an imaging detector for spatially resolved acquisition of an emission radiation emitted by an object to be examined; an illumination beam path between the illumination means and the object to be examined, and a detection beam path between the object to be examined and the detector, wherein the illumination beam path comprises illumination optics which are designed to generate a light sheet of illumination radiation extending transverse to the axis of the illumination beam path, wherein the axis of the detection beam path is oriented at an angle diverging from 0°
to a section plane of the light sheet and of the object to be examined, andwherein the illumination means comprise a pulsed laser. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification