METHODS AND COMPOSITIONS FOR BONE HEALING BY PERIOSTIN

• US 20110033516A1
• Filed: 08/05/2010
• Published: 02/10/2011
• Est. Priority Date: 08/06/2009
• Status: Abandoned Application

• Current Assignee: Medical University of South Carolina (State of South Carolina)
• Original Assignee: Medical University of South Carolina (State of South Carolina)
• Inventors: Kern, Michael J., Norris, Russell A., Markwald, Roger R., Kokko, Kyle P.
• Application Number:
  US12/851,100
• Publication Number:
  US 20110033516A1
• Time in Patent Office: Days
• US Class Current: 424/422
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 35/32 Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
• CPC Class Codes: A61K 38/1709 from mammals
• CPC Class Codes: A61K 38/39 Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
• CPC Class Codes: A61K 45/06 Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• CPC Class Codes: A61K 9/0021 Intradermal administration, e.g. through microneedle arrays, needleless injectors mechanical aspects A61M
• CPC Class Codes: A61L 2300/25 Peptides having up to 20 amino acids in a defined sequence
• CPC Class Codes: A61L 2300/252 Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
• CPC Class Codes: A61L 2300/412 Tissue-regenerating or healing or proliferative agents
• CPC Class Codes: A61L 2430/02 for reconstruction of bones; weight-bearing implants
• CPC Class Codes: A61L 27/227 Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
• CPC Class Codes: A61L 27/54 Biologically active materials, e.g. therapeutic substances A61L27/227 takes precedence
• CPC Class Codes: A61P 19/00 Drugs for skeletal disorders

Abstract

The present invention provides methods and compositions for increasing bone production and/or decreasing bone fracture healing time in a subject, by administering an effective amount of periostin and/or active peptides and/or fragments thereof.

Claims

1. A method of increasing bone production in a subject, comprising administering to the subject an effective amount of a periostin protein or a biologically active fragment thereof and/or a periostin peptide, thereby increasing bone production in the subject.

3. The method of claim 1, wherein the periostin protein or biologically active fragment thereof and/or peptide is administered directly to an injury site, wound site and/or surgical site in the subject.

5. The method of claim 1, wherein the periostin protein or biologically active fragment thereof and/or peptide is administered to the subject intravenously, orally and/or transdermally.

7. The method of claim 1, wherein the effective amount of the periostin protein or biologically active fragment thereof or the peptide is in the range of about 1 microgram/ml to about 500 milligrams/ml.

9. The method of claim 1, further comprising administering to the subject an agent selected from the group consisting of:

a) collagen;

b) a hydrogel;

c) a demineralized bone matrix;

d) an organic sponge;

e) an implantable matrix;

f) a bone chip; and

g) any combination of (a)-(f) above.

11. The method of claim 1, further comprising administering to the subject an agent selected from the group consisting of:

a) a differentiation stimulating agent;

b) a chemotaxis stimulating agent;

c) a proliferation stimulating agent;

d) a mobilization stimulating agent; and

e) any combination of (a)-(d) above.

13. The method of claim 9, further comprising administering to the subject an agent selected from the group consisting of:

a) a differentiation stimulating agent;

b) a chemotaxis stimulating agent;

c) a proliferation stimulating agent;

d) a mobilization stimulating agent; and

e) any combination of (a)-(d) above.

2. A method of decreasing healing time of a bone fracture in a subject in need thereof, comprising administering to the subject an effective amount of a periostin protein or a biologically active fragment thereof and/or a periostin peptide, thereby decreasing healing time of a bone fracture in the subject.

4. The method of claim 2, wherein the periostin protein or biologically active fragment thereof and/or peptide is administered directly to an injury site, wound site and/or surgical site in the subject.

6. The method of claim 2, wherein the periostin protein or biologically active fragment thereof and/or peptide is administered to the subject intravenously, orally and/or transdermally.

8. The method of claim 2, wherein the effective amount of the periostin protein or biologically active fragment thereof or the peptide is in the range of about 1 microgram/ml to about 500 milligrams/ml.

10. The method of claim 2, further comprising administering to the subject an agent selected from the group consisting of:

a) collagen;

b) a hydrogel;

c) a demineralized bone matrix;

d) an organic sponge;

e) an implantable matrix;

f) a bone chip; and

g) any combination of (a)-(f) above.

12. The method of claim 2, further comprising administering to the subject an agent selected from the group consisting of:

a) a differentiation stimulating agent;

b) a chemotaxis stimulating agent;

c) a proliferation stimulating agent;

d) a mobilization stimulating agent; and

e) any combination of (a)-(d) above.

14. The method of claim 10, further comprising administering to the subject an agent selected from the group consisting of:

a) a differentiation stimulating agent;

b) a chemotaxis stimulating agent;

c) a proliferation stimulating agent;

d) a mobilization stimulating agent; and

e) any combination of (a)-(d) above.