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Three-Dimensional Microfabricated Bioreactors with Embedded Capillary Network

  • US 20110033887A1
  • Filed: 09/24/2008
  • Published: 02/10/2011
  • Est. Priority Date: 09/24/2007
  • Status: Abandoned Application
First Claim
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1. A method of making a microvascularized bioreactor, said method comprising:

  • a. providing a photocurable liquid composition having a top surface;

    b. providing a source of light capable of curing at least a portion of said composition;

    c. illuminating said composition top surface with said light, wherein said illumination is in a pattern thereby simultaneously generating a polymerized pattern layer having a layer thickness;

    d. immersing said polymerized pattern layer into said composition depth by a vertical displacement corresponding to said layer thickness;

    e. waiting a surface dwell time for said surface to become substantially level;

    f. repeating said illuminating step to generate an adjacent polymerized pattern layer, wherein said repeating step is repeated for a number of steps to generate a microvascular network having an interior surface and an exterior surface; and

    g. contacting said exterior surface with a cell population, thereby obtaining said microvascularized bioreactor;

    wherein said microvascular network has a permeability to a biological material that varies with location within said network.

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