Multi-Channel Bio Reactor with Fluorescence Detector and On-Line Monitoring Apparatus of It

• US 20110035160A1
• Filed: 04/08/2009
• Published: 02/10/2011
• Est. Priority Date: 04/08/2008
• Status: Abandoned Application

• Current Assignee: Industry Foundation Of Chonnam National University (Chonnam National University)
• Original Assignee: Industry Foundation Of Chonnam National University (Chonnam National University)
• Inventors: Rhee, Jong IL, Sohn, Ok-Jae
• Application Number:
  US12/936,650
• Publication Number:
  US 20110035160A1
• Time in Patent Office: Days
• US Class Current: 702/22
• CPC Class Codes: C12M 23/12 Well or multiwell plates C12M25/04 takes precedence
• CPC Class Codes: C12M 23/42 Integrated assemblies, e.g. cassettes or cartridges
• CPC Class Codes: C12M 27/16 Vibrating; Shaking; Tilting
• CPC Class Codes: C12M 41/00 Means for regulation, monitoring, measurement or control, e.g. flow regulation controlling or regulating chemical, physical or physicochemical processes B01J19/0006; heating or cooling apparatus for laboratory use B01L7/00; electro optical investigation of individual particles, flow cytometers G01N15/14; automatic analysis G01N35/00; controlling or regulating in general G06N
• CPC Class Codes: G01N 2021/7786 Fluorescence
• CPC Class Codes: G01N 2021/7793 Sensor comprising plural indicators
• CPC Class Codes: G01N 21/77 by observing the effect on a chemical indicator

Abstract

Disclosed is a multi-channel bio reactor with fluorescence detector which measures various bio-chemical materials using fluorescence analysis, and an on-line monitoring apparatus of it. The multi-channel bio reactor with fluorescence detector comprises a light source 10 for emitting excitation light; a light irradiation part 30 for irradiating the excitation light emitted from the light source 10; a sample receiving part 40 in which the excitation light irradiated from the light irradiation part 30 is irradiated on a sample and which has a plurality of walls 41 for receiving a sample containing a fluorescent material; and an optical detecting part 50 for detecting fluorescence emitted from the sample received in the wall 41 of the sample receiving part 40.

Claims

1. A multi-channel fluorescence detector 100, comprising:

a light source 10 for emitting excitation light;

a light irradiation part 30 for irradiating the excitation light emitted from the light source 10;

a sample receiving part 40 in which the excitation light irradiated from the light irradiation part 30 is irradiated on a sample and which has a plurality of walls 41 for receiving a sample containing a fluorescent material; and

an optical detecting part 50 for detecting fluorescence emitted from the sample received in the wall 41 of the sample receiving part 40.

2. The multi-channel fluorescence detector according to claim 1, wherein the light irradiation part 30 is provided with a block 20 which is formed into one body, and comprises a light source coupling port 31 which is coupled with the light source 10, an excitation light irradiating path 32 through which excitation light emitted from the light source 10 is transmitted from the light source coupling port 31, and a band pass filter 33 which is coupled to an end or an internal portion of the excitation light irradiating path 32 placed at an inside portion of the light source 10 so as to pass only the excitation light having a special wavelength emitted from the light source 10.

3. The multi-channel fluorescence detector according to claim 2, wherein the optical detecting part 50 comprises a fluorescence path 51 which is provided at the block 20 of the light irradiation part 30 and through which the fluorescence emitted from the sample received in the well 41 of the sample receiving part 40 is transmitted, a band pass filter 53 which is disposed at the fluorescence path 51, and a detecting sensor 52 which is coupled to an end of the fluorescence path 51 so as to detect the transmitted fluorescence.

4. The multi-channel fluorescence detector according to claim 2, wherein the light source coupling port 31 and the excitation light irradiating path 32 are provided in plural so as to entirely or independently irradiate the excitation light.

5. The multi-channel fluorescence detector according to claim 2, wherein the excitation light irradiating path 32 is formed at the surface of the block adjacent to the sample receiving part 40 so as to form an angle of 0 to 90°

with respect to the light source coupling port 31.

6. The multi-channel fluorescence detector according to claim 2, wherein the block 20 is disposed at a lower side of the sample receiving part 40, and a fluorescent sensor film 42 for passing only particular fluorescence emitted from the sample or a fluorescent material for sensing the fluorescence is attached to or coated on a bottom surface of the well 41.

7. The multi-channel fluorescence detector according to claim 6, wherein the fluorescent sensor film 42 is divided into or mixed with n kinds or more (n=1) so as to measure various characteristics of biochemical materials.

8. The multi-channel fluorescence detector according to claim 7, wherein the fluorescence path 51 and the detecting sensor 52 of the optical detecting part 50 is comprised of a single detector or a plurality of fluorescent sensor films 42 in which the fluorescent dye is divided or mixed so as to detect the fluorescence.

9. The multi-channel fluorescence detector according to claim 2, wherein the block 20 is selectively disposed at a side portion, a lower portion and an upper portion of the sample receiving part 40 so as to irradiate the excitation light and detect the fluorescence.

10. The multi-channel fluorescence detector according to claim 3, wherein the detecting sensor 52 comprises PMT (Photo multiplier tube), PD (photo diode) and CCD (Charged couple device).

11. The multi-channel fluorescence detector according to claim 3, wherein an optical fiber is inserted into the excitation light irradiating path 32 or the fluorescence path 51.

12. The multi-channel fluorescence detector according to claim 11, wherein an end of the optical fiber is worked into an ellipse shape so as to increase an incident amount of the excitation light or the fluorescence.

13. The multi-channel fluorescence detector according to claim 11, further comprising a dichromatic mirror 54 which allows to irradiate the excitation light and detect the fluorescence using a single piece of the optical fiber.

14. The multi-channel fluorescence detector according to claim 2, wherein the block 20 is fixed to a moving table 61 so as to move left and right or forward and backward, so that the light irradiation part 30 and the optical detecting part 50 irradiate the light on the well 41 or detect the light from the well 41.

15. A multi-channel fluorescence detector on-line monitoring apparatus, comprising:

a main body 110 in which a multi-channel fluorescence detector 100 according to claim 1 is provided;

a data transmitting part 120 which transmits data detected from the optical detecting part 50 of the multi-channel fluorescence detector 100 and receives a control signal for the multi-channel fluorescence detector 100;

a data receiving part 130 which receives data transmitted from the data transmitting part 120 and transmits the control signal for the multi-channel fluorescence detector 100; and

a micro-processor 140 which analyzes and stores the received data received from the data receiving part 130 and controls the multi-channel fluorescence detector 100.

16. The multi-channel fluorescence detector on-line monitoring apparatus according to claim 15, wherein the main body 110 is formed with an air inlet port 112 through which external air is introduced and an air outlet port 113 through which air in the main body 110 is discharged, and comprises a heater 116 which is provided at the air inlet port 112, an air inlet fan 114 which is disposed at a rear side of the heater 116 so as to suck air heated by the heater 116, an air inlet slot 151, 152 through which the air is introduced by the air inlet fan 114, an air outlet port 153 which is provided at an outer side of the air inlet slot 151, 152 and communicated with the air inlet slot 151, 152 so as to discharge the heated air inside the main body 110, and an air outlet fan 115 which is provided at the air outlet port 113 so as to discharge air in the main body 110 to an outside, and the main body 110 further comprises a thermostat 150 for constantly maintaining an internal temperature in the main body 110.

17. The multi-channel fluorescence detector on-line monitoring apparatus according to claim 16, further comprising an antifungal filter 160 is provided at the air inlet port 112 and the air outlet port 113 so as to filter internal air discharged from the main body 110.

18. The multi-channel fluorescence detector on-line monitoring apparatus according to claim 16, further comprising a vibrator 170 which vibrates the sample receiving part 40 of the multi-channel fluorescence detector and thus stirs the sample received in the sample receiving part 40.

19. The multi-channel fluorescence detector on-line monitoring apparatus according to claim 17, further comprising a vibrator 170 which vibrates the sample receiving part 40 of the multi-channel fluorescence detector and thus stirs the sample received in the sample receiving part 40.