BIOSENSOR

• US 20110036713A1
• Filed: 10/20/2010
• Published: 02/17/2011
• Est. Priority Date: 06/24/2005
• Status: Active Grant

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Akihisa Higashihara, Eriko Yamanishi, Hiroyuki Tokunaga
• Inventors: Tokunaga, Hiroyuki, Higashihara, Akihisa, Yamanishi, Eriko
• Granted Patent: US 8,038,860 B2
• Time in Patent Office: Days
• US Class Current: 204/403.01
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52

Abstract

A biosensor can supply a sample solution accurately and easily, and includes a capillary for collecting a sample solution and analyzes a specific substance in the sample solution, an air hole, and at least two supply ports, i.e., a sample supply port and an auxiliary sample supply port, so that supply of the sample solution can be performed from either of the supply ports. Even when the sample supply port is closed up with a fingertip or the like and supply of the sample solution is stopped, the sample solution can be quickly supplied from the other auxiliary sample supply port.

Claims

1-12. -12. (canceled)

13. A biosensor comprising

a first insulating substrate and a second insulating substrate bonded together;

a sample supply port which opens at one end of the bonded insulating substrates, and to which a sample solution is applied;

a capillary communicated with the sample supply port, and into which the applied sample solution is introduced;

an air hole which is positioned at an end of the capillary and communicated with air inside the capillary; and

a spacer having a groove, the spacer being disposed between the first insulating substrate and the second insulating substrate,

wherein said sample supply port and said capillary are formed by bonding the insulating substrates together,

wherein an auxiliary sample supply port communicated with said capillary, through which the applied sample solution is introduced into the capillary, is provided in the vicinity of said sample supply port,

wherein the groove in the spacer forms the capillary and the sample supply port, and

wherein the auxiliary sample supply port is disposed at one end of the bonded insulating substrates.

14. A biosensor as defined in claim 13, wherein a total aperture area of said auxiliary sample supply port is 0.01 mm² to 3 mm².

15. A biosensor as defined in claim 13, wherein a distance between said sample supply port and said auxiliary sample supply port is 0.05 to 5 mm.

16. A biosensor as defined in claim 13, wherein said through-hole for the auxiliary sample supply port is fabricated using a laser.

17. A biosensor as defined in claim 13, wherein a surface-activating treatment is applied to at least a portion of a surface of the first insulating substrate or the second insulating substrate, said portion facing the capillary.

18. A biosensor as defined in claim 13, wherein electrodes and a reagent layer for electrochemically analyzing a specific substance in the sample solution are provided on a surface of the first insulating substrate or the second insulating substrate, said surface facing the capillary.

19. A biosensor as defined in claim 18, wherein the first insulating substrate and the second insulating substrate have different shapes at an end of the biosensor where the sample supply port is formed.

20. A biosensor as defined in claim 19, wherein the electrodes or the reagent layer are/is not provided on the first or second insulating substrate which is opposed to the auxiliary sample supply port.

21. A biosensor as defined in claim 13, wherein a surface-activating treatment is applied to an inner wall of the auxiliary sample supply port.

22. A biosensor as defined in claim 13, wherein said auxiliary supply port is a first auxiliary supply port, and the biosensor comprises at least on additional auxiliary supply port, and

wherein a total aperture area of the auxiliary sample supply ports is 0.01 mm² to 3 mm².

23. A biosensor as defined in claim 13, wherein said auxiliary sample supply port is disposed between said air hole and said sample supply port.

24. A biosensor as defined in claim 13, wherein said auxiliary supply port is a first auxiliary supply port and the biosensor includes at least one additional auxiliary supply port, and

wherein each of said auxiliary sample supply ports is disposed between said air hole and said sample supply port.

25. A biosensor as defined in claim 13, wherein said auxiliary sample supply port and said air hole are disposed in said second insulating substrate, and

wherein said auxiliary sample supply port is disposed between said air hole and said sample supply port.

26. A biosensor as defined in claim 13, wherein said auxiliary sample supply port is formed in one of said insulating substrates, said sample supply port is formed between said insulating substrates, and said air hole is formed in one of said insulating substrates.

27. A biosensor as defined in claim 13, further comprising a reagent layer disposed between the spacer and the second insulating substrate, the reagent layer being in fluid communication with the capillary.