Microchip

• US 20110038758A1
• Filed: 11/18/2005
• Published: 02/17/2011
• Est. Priority Date: 11/22/2004
• Status: Abandoned Application

• Current Assignee: Nissui Pharmaceutical Co. Ltd. (Shimadzu Corporation)
• Original Assignee: Nissui Pharmaceutical Co. Ltd. (Shimadzu Corporation)
• Inventors: Tokeshi, Manabu, Oku, Yuichi, Kitamori, Takehiko, Akaba, Shuichi
• Application Number:
  US11/791,102
• Publication Number:
  US 20110038758A1
• Time in Patent Office: Days
• US Class Current: 422/69
• CPC Class Codes: B01L 2200/0668 Trapping microscopic beads
• CPC Class Codes: B01L 2300/0627 Sensor or part of a sensor is integrated
• CPC Class Codes: B01L 2300/0825 Test strips
• CPC Class Codes: B01L 2300/0877 Flow chambers
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: B81B 1/002 Holes characterised by their shape, in either longitudinal or sectional plane
• CPC Class Codes: B81B 2201/058 Microfluidics not provided for in B81B2201/051 - B81B2201/054
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing

Abstract

A microchip, which is used in a diagnostic system using a microfluid system, has a flow path capable of greatly improving the reaction efficiency and realizing a stable measurement with high reproducibility. The microchip has two substrates with at least a flow path 12 formed at the interface between the two substrates, the flow path 12 having a reaction area 14 and a detection area 15 downstream of the reaction area 14, and the flow path 12 at the detection area 15 having a depth which is deeper than the flow path 12 in the reaction area 14.

Claims

1. A microchip comprising two substrates having at least a flow path formed at the interface between the two substrates, the flow path having a reaction area and a detection area downstream of the reaction area, and the flow path in the detection area having a depth which is deeper than the flow path in the reaction area.

10. The microchip according to claim 1 wherein said reaction area has an interface which contributes to the reaction and said interface is at least a part of the interface of the flow path.

11. The microchip according to claim 1 wherein the microchip is used for measuring a biological substance, and said interface contributing to the reaction has immobilized thereon an anti-biological substance which is capable of binding to the biological substance to be measured by affinity to thereby capture said biological substance.

12. The microchip according to claim 11 wherein a nucleic acid is immobilized on the interface of the flow path, and said anti-biological substance is bonded to the nucleic acid.

13. The microchip according to claim 11 wherein magnetic particles are immobilized on the interface of the flow path by magnetism of a magnet provided in or outside the flow path, and said anti-biological substance is immobilized on the surface of the magnetic particles.

14. The microchip according to claim 1 wherein the flow path at the reaction are has a width of 1 mm to 2 mm.

15. The microchip according to claim 1 wherein the flow path at the reaction are has a width of 1 mm to 500 mm.

16. The microchip according to claim 1 wherein the flow path at the detection are has a depth of 10 mm to 2 mm.

2. A microchip comprising two substrates having at least a flow path formed at the interface between the two substrates, the flow path having a reaction area and a detection area downstream of the reaction area, the flow path in the reaction area having a width which is wider than the flow path at the detection area, and the flow path at the detection area having a depth which is deeper than the flow path in the reaction area.

3. The microchip according to claim 2 wherein said reaction area has an interface which contributes to the reaction and said interface is at least a part of the interface of the flow path.

4. The microchip according to claim 2 wherein the microchip is used for measuring a biological substance, and said interface contributing to the reaction has immobilized thereon an anti-biological substance which is capable of binding to the biological substance to be measured by affinity to thereby capture said biological substance.

5. The microchip according to claim 4 wherein a nucleic acid is immobilized on the interface of the flow path, and said anti-biological substance is bonded to the nucleic acid.

6. The microchip according to claim 4 wherein magnetic particles are immobilized on the interface of the flow path by magnetism of a magnet provided in or outside the flow path, and said anti-biological substance is immobilized on the surface of the magnetic particles.

7. The microchip according to claim 2 wherein the flow path at the reaction are has a width of 1 mm to 2 mm.

8. The microchip according to claim 2 wherein the flow path at the reaction are has a width of 1 mm to 500 mm.

9. The microchip according to claim 2 wherein the flow path at the detection are has a depth of 10 mm to 2 mm.