Apparatus and Process for Generating and Harvesting Adult Stem Cells and Fluid Associated with it from Skin and Omentum for Medical, Cosmetic, and Veterinary Use
First Claim
1. A granuloma fluid collection apparatus implantable under skin of a donor comprising a piece of non-toxic material having walls forming an enclosed chamber, wherein the walls include a plurality of holes therethrough, whereby a fluid rich in natural growth factors forms within the granuloma fluid collection apparatus by steady diffusion from the granulation tissue surrounding the apparatus through the plurality of holes for entrapment within the enclosed chamber for harvesting or collecting.
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Accused Products
Abstract
Apparatus and process to create, expand, and localize the population of stem cells in adult tissues by placing a foreign body either in omentum or in the subcutaneous tissue. The apparatus 1) traps the granulation fluid, cells a fluid rich in a myriad of growth factors, 2) mobilizes and concentrates free-floating stem cells in the granulation fluid, and 3) stimulates the growth of a new tissue around the apparatus, which provides another source of stem cells. Stem cells obtained by this process can be used as freshly isolated or after culturing, which can be used for wound healing, treating diseases, and regenerating organs in animals and humans. The granulation fluid could substitute the stem cells in many of their applications and offers the convenience of an injectable medicine for treating animal and human diseases.
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Citations
20 Claims
- 1. A granuloma fluid collection apparatus implantable under skin of a donor comprising a piece of non-toxic material having walls forming an enclosed chamber, wherein the walls include a plurality of holes therethrough, whereby a fluid rich in natural growth factors forms within the granuloma fluid collection apparatus by steady diffusion from the granulation tissue surrounding the apparatus through the plurality of holes for entrapment within the enclosed chamber for harvesting or collecting.
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6. The method of harvesting a fluid rich in natural growth factors comprising the steps of:
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providing a non-toxic container having an enclosed chamber formed by walls with through-holes; implanting the non-toxic container under skin of a donor such that a granulation tissue forms about an exterior surface of the non-toxic container that secretes a growth factor rich fluid through the through-holes of the non-toxic container and into the enclosed chamber of the non-toxic container; and removing the granuloma fluid from the enclosed chamber of the non-toxic container, wherein the non-toxic container traps a liquid which is high in a myriad of growth factors used for the production of adult stem cells. - View Dependent Claims (7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
(ii) as a piece of tissue for organ repair for heart valve, joint, intervertibral disc and the like, as a surgical filler tissue for filling tissue lost from ischemic or other injury, or as an artificial skin in cases of third degree burns, or as a piece of detached pedicle for placing on injured organs, such as spinal cord, to induce angiogenesis and accelerate wound healing; (iii) local or systemic injections containing freshly extracted individual cells or cultured stem cells for treatment of acute and chronic diseases;
or(iv) manufacture of purified growth factors from the culture medium of cultured cells.
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17. A product of the process according to claim 6, wherein the product is a granuloma fluid powder produced in the skin by a foreign body comprising the step of freeze drying the granuloma fluid.
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18. The product according to claim 17, wherein the granuloma powder is reconstituted into a vaccine comprising the steps of:
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adding a measured amount of sterile water to a vial containing granuloma fluid powder; shaking the vial to re-suspend the preparation; waiting for a predetermined time to allow complete dissolution; and taking the preparation in the injection syringe for an injection in the patient.
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19. The product according to claim 18, wherein the measured amount of sterile water is 1 mL, the vial contains approximately 50 milligrams of proteins including albumin and growth factors, and the predetermined time is 1-2 minutes to prepare the injection of 1 mL.
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20. The product according to claim 18, wherein the measured amount of sterile water is 5 mL, the vial contains 250 milligrams of proteins including albumin and growth factors, and the predetermined time is 1-2 minutes to prepare the injection of 5 mL.
Specification