FLOWCELL SYSTEMS FOR SINGLE MOLECULE DETECTION
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Abstract
The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charge-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electrically sorting charged molecules, the cleaved product molecules are detected in isolation without interference from unincorporated NPs and without illuminating the polymerase-DNA complex.
92 Citations
70 Claims
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1-48. -48. (canceled)
- 49. A nucleotide, said nucleotide having a detectable label attached to the terminal phosphate, wherein said detectable label is linked to said terminal phosphate with a linker comprising a peptide of 2 to 15 amino acids in length.
Specification