TRACKING AND CHARACTERIZING PARTICLES WITH HOLOGRAPHIC VIDEO MICROSCOPY
1 Assignment
0 Petitions
Accused Products
Abstract
In-line holography to create images of a specimen, such as one or more particles dispersed in a transparent medium. Analyzing these images with results from light scattering theory yields the particles'"'"' sizes with nanometer resolution, their refractive indexes to within one part in a thousand, and their three dimensional positions with nanometer resolution. This procedure can rapidly and directly characterize mechanical, optical and chemical properties of the specimen and its medium.
-
Citations
60 Claims
-
1-35. -35. (canceled)
-
36. A method for characterizing a specimen, comprising:
-
providing a holographic microscope; providing a collimated laser beam to the holographic microscope; scattering the collimated laser beam off the specimen to generate a scattered portion; generating an interference pattern from an unscattered portion of the collimated laser beam and the scattered portion; recording the interference pattern for subsequent analysis; and measuring at least one of a spatial location, a mechanical property, an optical property and a chemical property of the specimen. - View Dependent Claims (37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49)
-
-
50. A method for optically characterizing a specimen disposed in a medium, including the steps of providing a holographic microscope, providing a laser beam to the holographic microscope, scattering a collimated laser beam off the specimen in the medium to generate a scattered portion;
- generating an interference pattern from an unscattered portion of the collimated laser beam and the scattered portion;
recording the interference pattern for subsequent analysis; and
measuring at least one of a spatial location of the specimen, a mechanical property of the specimen, an optical property of the specimen, a chemical property of the specimen, and a characteristic interaction between the specimen and the medium and a feature of the medium. - View Dependent Claims (51, 52, 53, 54, 55)
- generating an interference pattern from an unscattered portion of the collimated laser beam and the scattered portion;
-
56. A computer readable medium for processing information to characterize a specimen comprising,
a computer software module including computer software having a set of instructions for operating on non-transitory data characteristic of the specimen; -
the non-transitory data characteristic of the specimen related to an interference pattern arising from interaction of a laser beam scattered from the specimen and an unscattered portion of the laser beam; and the set of instructions further including a scattering function which upon execution provides non-transitory convergent solution data descriptive of properties of the specimen, thereby enabling characterization of at least one of a mechanical property, an optical property, and a chemical property of the specimen. - View Dependent Claims (57, 58, 59, 60)
-
Specification