Toxic Material Detection Apparatus and Method

• US 20110045517A1
• Filed: 11/06/2008
• Published: 02/24/2011
• Est. Priority Date: 11/06/2007
• Status: Abandoned Application

• Current Assignee: Battelle Memorial Institute
• Original Assignee: Battelle Memorial Institute
• Inventors: Clark, David N, Danison, Tom, Black, Rodney S, Petrel, Trevor, Slivon, Laurence, Moore, Fred, Derringer, Tricia L, Shaw, Matthew J
• Application Number:
  US12/741,761
• Publication Number:
  US 20110045517A1
• Time in Patent Office: Days
• US Class Current: 435/20
• CPC Class Codes: C12Q 1/46 involving cholinesterase
• CPC Class Codes: G01N 21/25 Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
• CPC Class Codes: G01N 21/78 producing a change of colour

Abstract

A toxic material detection apparatus includes a sample collection portion including a sample inlet and a sample concentrator adapted to concentrate an environmental sample on a substrate. A sample distributing system transfers portions of the substrate to a color sensor and an ion mobility spectrometer for simultaneously analysis and toxin detection, particularly cholinesterase inhibitor detection. Optionally, a portion of the substrate may be directed to an archive for possible analysis at a later time. Reagents utilized include the enzyme acetylcholinesterase (AChE), and the reactants acetylthiocholine iodide (ATCI) and 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTB). A data management unit provides for near-real-time analysis of the samples in under 5 minutes. Simultaneous “hits” by both analysis methods indicate the presence of a cholinesterase inhibitor.

Claims

1. A toxic material detection apparatus comprising:

a sample collection portion including a sample inlet and a sample concentrator adapted to concentrate an environmental sample on a substrate;

a color sensor adapted to analyze the substrate for a presence of toxic materials;

a spectrometer separate and distinct from the color sensor and adapted to analyze the substrate for a presence of toxic materials;

a sample distributing system adapted to distribute the environmental sample to the color sensor and the spectrometer simultaneously; and

a data management unit in communication with the color sensor and spectrometer.

2. The apparatus of claim 1 further comprising:

an archive portion, wherein the sample distributing system is adapted to distribute the environmental sample to both the color sensor and the spectrometer, as well as to the archive portion for possible later analysis.

3. The apparatus of claim 1, wherein the sample distributing system is in the form of a reel-to-reel system and the substrate is in the form of a substrate tape.

4. The apparatus of claim 3, wherein the distributing system includes a first reel of layered substrate material including first and second layers;

a second reel in communication with the spectrometer and connected to the first layer of the substrate material; and

a third reel in communication with the color detector sensor and connected to the second layer of the substrate material.

5. The detection system of claim 4, further comprising:

an archive portion, wherein the sample distributing system further comprises a fourth reel in communication with the archive portion and connected to a third layer of the substrate material.

6. The apparatus of claim 3, wherein the substrate is constituted by a cloth or membrane.

7. The apparatus of claim 6, wherein the substrate is selected from the group consisting of a polyester felt, a Nomex felt or a fabric material.

8. The apparatus of claim 1, wherein the sample collection portion is a high volume aerosol collection system including a dry cyclone collector.

9. The apparatus of claim 8, wherein the collection system is adapted to operate in batch mode.

10. The apparatus of claim 9, wherein the substrate is in the form of a filter material.

11. The apparatus of claim 1, wherein the sample collection portion includes a pump adapted to draw ambient air into the sample inlet such that the ambient air impinges on the substrate.

12. The apparatus of claim 1, wherein the sample collection portion includes a wet cyclone collector.

13. The apparatus of claim 1, further comprising:

a reagent applicator for applying one or more reagents to the substrate.

14. The apparatus of claim 1, wherein the substrate is a pretreated substrate including one or more reagents thereon.

15. The apparatus of claim 1, further comprising:

a sample port; and

a heater interposed between the sample port and the spectrometer.

16. The apparatus of claim 1, wherein the spectrometer is an ion mobility spectrometer.

17. A method for detecting a toxic material comprising:

impinging ambient air onto a substrate to concentrate any airborne aerosols on the substrate;

distributing a first portion of the substrate to a color sensor and simultaneously distributing a second portion of the substrate to a spectrometer which is separate and distinct from the color sensor;

detecting a presence of toxic materials on the first portion of the substrate utilizing the color sensor;

detecting a presence of toxic materials on the second portion of the substrate utilizing the spectrometer; and

determining whether toxic materials are present on the substrate based on both the presence of toxic materials detected utilizing the color sensor and the presence of toxic materials detected utilizing the spectrometer.

18. The method of claim 17, wherein determining the presence of toxic materials is conducted electronically.

19. The method of claim 17, wherein determining the presence of toxic materials is conducted manually.

20. The method of claim 17, wherein the color sensor detects the presence of toxic materials based on cholinesterase inhibition.

21. The method of claim 20, further comprising:

adding the reagents acetylcholinesterase, 5,5′

-dithio-bis-(2-nitrobenzoic acid) and acetylthiocholine iodide to the substrate prior to determining the presence of toxic materials on the substrate.

22. The method of claim 17, wherein the aerosols are deposited on the substrate through high volume filtration.

23. The method of claim 17, wherein the aerosols are deposited through high volume impaction.

24. The method of claim 17, further comprising:

distributing a third portion of the substrate to an archive portion for possible later analysis.