High Throughput Screen
First Claim
1. An apparatus for use with a biological cellular membrane in detecting and assaying compounds with ion channel activity, comprising:
- a well with a porous substrate disposed therein, said substrate having at least one pore with a diameter between 0.5 μ
m and 10 μ
m;
a solution perfusion channel disposed below said porous substrate and defining an inlet and an outlet for flow of fluid therethrough;
a first electrode disposed in said well; and
a second electrode disposed in said solution perfusion channel in electrical communication with the first electrode through said porous substrate
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Accused Products
Abstract
The present invention relates to a structure comprising a biological membrane and a porous or perforated substrate, a biological membrane, a substrate, a high throughput screen, methods for production of the structure membrane and substrate, and a method for screening a large number of test compounds in a short period. More particularly it relates to a structure comprising a biological membrane adhered to a porous or perforated substrate, a biological membrane capable of adhering with high resistance seals to a substrate such as perforated glass and the ability to form sheets having predominantly an ion channel or transporter of interest, a high throughput screen for determining the effect of test compounds on ion channel or transporter activity, methods for manufacture of the structure, membrane and substrate, and a method for monitoring ion channel or transporter activity in a membrane.
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Citations
20 Claims
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1. An apparatus for use with a biological cellular membrane in detecting and assaying compounds with ion channel activity, comprising:
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a well with a porous substrate disposed therein, said substrate having at least one pore with a diameter between 0.5 μ
m and 10 μ
m;a solution perfusion channel disposed below said porous substrate and defining an inlet and an outlet for flow of fluid therethrough; a first electrode disposed in said well; and a second electrode disposed in said solution perfusion channel in electrical communication with the first electrode through said porous substrate - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. An apparatus, comprising:
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a plate defining a plurality of wells; a porous substrate disposed in a bottom of each said well, wherein the substrate in each well defines at least one pore with a diameter between 1 μ
m and 7 μ
m, and is configured and dimensioned to permit adherence of a biological cellular membrane on an upper side thereof with a high resistance seal between the membrane and said porous substrate having sufficient electrical resistance to facilitate patch clamping measurements across a biological membrane adhered to said substrate for detecting ion channel activity in the membrane;a solution perfusion channel disposed below the porous substrate of each said well, said channel defining an inlet for flow of fluid therethrough across said pores and an outlet; at least a first electrode disposable in each said well; and at least a second electrode disposed in said solution perfusion channel in electrical communication with said first electrode across the porous substrate. - View Dependent Claims (17, 18, 19, 20)
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Specification