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Producing Itaconic Acid in Yeast Using Glycerol as the Substrate

  • US 20110053232A1
  • Filed: 08/25/2009
  • Published: 03/03/2011
  • Est. Priority Date: 08/25/2009
  • Status: Active Grant
First Claim
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1. A method of producing itaconic acid in yeast, comprisingproviding a genetically modified yeast host cell, the host cell containing a first expression cassette including a first yeast promoter operably linked to a nucleotide sequence encoding a cis-aconitic acid decarboxylase,culturing the yeast host cell in a medium containing glycerol at a concentration of 5-700 g/L, wherein the yeast host cell converts glycerol to itaconic acid, and collecting the medium for isolation of itaconic acid.

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