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METHODS AND COMPOSITIONS FOR POLYPEPTIDE ENGINEERING

  • US 20110053781A1
  • Filed: 09/11/2009
  • Published: 03/03/2011
  • Est. Priority Date: 12/18/1996
  • Status: Abandoned Application
First Claim
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1. A method for evolving a protein encoded by a DNA substrate molecule comprising:

  • (a) digesting at least a first and second DNA substrate molecule, wherein the at least a first and second substrate molecules differ from each other in at least one nucleotide, with a restriction endonuclease;

    (b) ligating the mixture to generate a library of recombinant DNA molecules;

    (c) screening or selecting the products of (b) for a desired property; and

    (d) recovering a recombinant DNA substrate molecule encoding an evolved protein.

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