METHODS AND COMPOSITIONS FOR POLYPEPTIDE ENGINEERING
First Claim
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1. A method for evolving a protein encoded by a DNA substrate molecule comprising:
- (a) digesting at least a first and second DNA substrate molecule, wherein the at least a first and second substrate molecules differ from each other in at least one nucleotide, with a restriction endonuclease;
(b) ligating the mixture to generate a library of recombinant DNA molecules;
(c) screening or selecting the products of (b) for a desired property; and
(d) recovering a recombinant DNA substrate molecule encoding an evolved protein.
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Abstract
Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.
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Citations
2 Claims
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1. A method for evolving a protein encoded by a DNA substrate molecule comprising:
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(a) digesting at least a first and second DNA substrate molecule, wherein the at least a first and second substrate molecules differ from each other in at least one nucleotide, with a restriction endonuclease; (b) ligating the mixture to generate a library of recombinant DNA molecules; (c) screening or selecting the products of (b) for a desired property; and (d) recovering a recombinant DNA substrate molecule encoding an evolved protein.
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2-273. -273. (canceled)
Specification