SYSTEM FOR MIXING FLUIDS BY COALESCENCE OF MULTIPLE EMULSIONS
First Claim
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1. A method of sample analysis, comprising:
- obtaining a plurality of compound droplets each including at least one sample-containing droplet, at least one reagent-containing droplet, and a barrier droplet that separates the sample-containing droplet from the reagent-containing droplet; and
fusing the sample-containing droplet and the reagent-containing droplet within individual compound droplets to form fused droplets that combine sample and reagent to create assay mixtures for performing an assay.
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Abstract
System, including methods, apparatus, compositions, and kits, for the mixing of small volumes of fluid by coalescence of multiple emulsions.
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Citations
29 Claims
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1. A method of sample analysis, comprising:
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obtaining a plurality of compound droplets each including at least one sample-containing droplet, at least one reagent-containing droplet, and a barrier droplet that separates the sample-containing droplet from the reagent-containing droplet; and fusing the sample-containing droplet and the reagent-containing droplet within individual compound droplets to form fused droplets that combine sample and reagent to create assay mixtures for performing an assay. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A composition for sample analysis, comprising:
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a plurality of compound droplets disposed in a continuous phase, each compound droplet including at least one sample-containing droplet, at least one reagent-containing droplet, and a barrier droplet that separates the sample-containing droplet from the reagent-containing droplet, wherein fusion of the sample-containing droplet and the reagent-containing droplet within individual compound droplets to form fused droplets is inducible by heating the compound droplets, and wherein the fused droplets combine sample and reagent to create assay mixtures for performing an assay. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23)
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24. A method of sample analysis, comprising:
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selecting an emulsion from a set of pre-formed emulsions each containing different reagent-containing droplets; forming compound droplets that each include at least one sample-containing droplet, at least one reagent-containing droplet from the selected emulsion, and a barrier droplet that separates the sample-containing droplet from the reagent-containing droplet; and fusing the sample-containing droplet and the reagent-containing droplet within individual compound droplets to form fused droplets that combine reagent from the selected emulsion with sample to create assay mixtures for performing an assay. - View Dependent Claims (25, 26, 27, 28, 29)
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Specification