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METHODS FOR SEQUENCE DETERMINATION

  • US 20110059436A1
  • Filed: 04/07/2009
  • Published: 03/10/2011
  • Est. Priority Date: 07/07/2000
  • Status: Abandoned Application
First Claim
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1. A system for determining sequence information at the single molecule level, comprising:

  • a detector comprising a viewing field,at least one active complex confined within the viewing field, where the complex comprises;

    a reaction buffer,a polymerizing agent,a template,a primer adapted to duplex with a portion of the template to form an extendable duplex, andmonomer types for the polymerizing agent,where the polymerizing agent and/or the monomer types comprising tags having detectable properties that undergo changes before, during and/or after a monomer incorporation event, where the tags and the detectable properties are same or different; and

    an excitation source adapted to excite the polymerizing agent and/or monomer tags within the viewing field,where the detector is adapted to detect changes in the detectable properties of the tags before, during and/or after one or a series of monomer incorporation events and to convert the incorporation event or events into an identity of one monomer or identities of a plurality of monomers complementary to a sequence of monomers in the template.

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