Laser Capture Microdissection (LCM) Extraction Device and Device Carrier, and Method for Post-LCM Fluid Processing

• US 20110059548A1
• Filed: 08/13/2010
• Published: 03/10/2011
• Est. Priority Date: 04/26/2000
• Status: Active Grant

• Current Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Head, David F., Richardson, Bruce J., Richardson, Derrick A., Moravick, Keith E., Baer, Thomas M., Reamey, Robert H.
• Granted Patent: US 9,103,757 B2
• Time in Patent Office: Days
• US Class Current: 436/178
• CPC Class Codes: G01N 1/2813 Producing thin layers of samples on a substrate, e.g. smearing, spinning-on G01N1/30 takes precedence
• CPC Class Codes: G01N 1/30 Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
• CPC Class Codes: G01N 1/40 Concentrating samples
• CPC Class Codes: G01N 2001/284 using local activation of adhesive, i.e. Laser Capture Microdissection
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

The present invention generally discloses an extraction system that provides a locale for fluid processing and extraction on a post-microcapture transfer film. The extraction system includes a transfer film carrier and an extraction device forming a reservoir. The extraction system selectively excludes regions of the transfer film from the reservoir to advantageously reduce contamination due to matter adhered to the transfer film by non-specific transfer.

Claims

1-21. -21. (canceled)

22. An extraction device for mating with a carrier comprising:

a carrier-receiving portion at a first end;

the carrier-receiving portion including a shoulder and at least one flange;

the at least one flange extending from the shoulder;

the carrier-receiving portion further including a landing portion having a landing surface;

the landing surface defining an inner opening; and

a conduit interconnected to the carrier-receiving portion at the inner opening;

the conduit extending between the carrier-receiving portion and a second end;

wherein the carrier-receiving portion is adapted to receive a carrier and to form a reservoir by the carrier contacting the landing portion.

23. The extraction device of claim 22 wherein the landing surface is raised from the shoulder.

25. The extraction device of claim 22 further including a rim at the first end;

the rim having a beveled surface adapted to guide the insertion of the carrier into the carrier-receiving portion.

26. The extraction device of claim 22 wherein the carrier-receiving portion includes four flanges;

each flange being curved to collectively encompass the carrier.

27. The extraction device of claim 22 further including securing features adapted to secure the carrier to the carrier-receiving portion.

28. The extraction device of claim 27 wherein the securing features are selected from the group consisting of a compression-fit engagement between the carrier and the extraction device, a snap-fit engagement between the carrier and the extraction device, a lock-and-key engagement between the carrier and the extraction device, and an adhesive engagement between the carrier and the extraction device.

29. The extraction device of claim 22 wherein the conduit is adapted to mate with a vessel at the second end.

31. The extraction device of claim 22 further including a gasket having a central aperture;

the gasket being disposed on the landing portion such that the central aperture coincides with the inner opening.

33. The extraction device of claim 22 wherein a fluidic barrier is formed with the carrier at the inner opening such that fluid is retained in the reservoir.

35. The extraction device of claim 22 wherein the reservoir is defined by the conduit and at least a portion of the carrier encompassed by the inner opening.

36. The extraction device of claim 35 wherein the carrier includes a transfer film coupled to a substrate surface;

the portion of the carrier encompassed by the inner opening including a portion of the transfer film such that the portion of the transfer film is disposed inside the reservoir.

37. The extraction device of claim 22 wherein the carrier includes at least one stand-off portion;

the stand-off portion being disposed outside the reservoir.

24. (canceled)

30. (canceled)

32. (canceled)

34. (canceled)

38-51. -51. (canceled)

52. An extraction device for mating with a carrier comprising:

a carrier-receiving portion at a first end;

the carrier-receiving portion including an inner surface, a landing portion, and a reservoir forming surface;

the landing portion being interconnected with the inner surface and the reservoir forming surface;

the reservoir forming surface being encompassed by the landing portion; and

at least one conduit interconnected to the carrier-receiving portion;

the conduit extending between the reservoir-forming surface and a second end;

wherein the carrier receiving portion is adapted to receive a carrier and to form a reservoir by the carrier contacting the landing portion.

53. The extraction device of claim 52 further including a recess interconnected with the inner surface and the landing portion.

54. The extraction device of claim 52 wherein the landing portion is raised from the reservoir-forming surface.

55-78. -78. (canceled)

79. A method for extracting matter on a carrier comprising the steps of:

providing a carrier having a transfer film;

transferring matter to the transfer film;

providing an extraction device;

mating the carrier to the extraction device;

forming a reservoir with the transfer film;

providing fluid into the reservoir to extract matter from the transfer film; and

removing the fluid from the reservoir.

80. The method of claim 79 wherein the step of transferring matter to the transfer film includes transferring matter to the transfer film by specific transfer microcapture;

and

further including the step of disposing matter that is adhered to the transfer film by specific transfer microcapture within the reservoir.

81. The method of claim 79 wherein the step of transferring matter to the transfer film includes transferring matter to the transfer film by non-specific transfer micro-capture;

and

further including the step of substantially excluding matter that is adhered to the transfer film by non-specific transfer microcapture from the reservoir.

82. The method of claim 79 wherein the step of providing a carrier having a transfer film includes providing a carrier with at least one stand-off portion;

and further including the step of covering the at least one stand-off portion.

83. A method of delivering at least one extraction device comprising the steps of:

providing an extraction device delivery system comprising a base; and

a locator connected to the base;

wherein the locator includes at least one aperture adapted to receive at least one extraction device;

providing at least one extraction device;

disposing the at least one extraction device within the at least one aperture;

providing a carrier having a transfer film;

passing the carrier through the locator such that the transfer film contacts the at least one extraction device;

adhering the carrier to the extraction device to form a carrier-extraction device combination; and

removing the carrier-extraction device combination.

84. The method of claim 83 wherein the step of providing an extraction device delivery system includes providing a guide connected to the locator.

86. The method of claim 83 wherein the step of providing an extraction device delivery system further includes providing a first adhesive layer between the base and the locator.

85. (canceled)

87-92. -92. (canceled)