Assay Plates, Reader Systems and Methods for Luminescence Test Measurements

• US 20110059870A1
• Filed: 11/11/2010
• Published: 03/10/2011
• Est. Priority Date: 06/29/2001
• Status: Active Grant

• Current Assignee: Meso Scale Technologies, LLC
• Original Assignee: Meso Scale Technologies, LLC
• Inventors: Wohlstadter, Jacob N., Kishbaugh, Alan, Jeffrey-Coker, Bandele, Wilbur, James, Fischer, Alan B., Debad, Jeff D., Glezer, Eli, Clinton, Charles, Johnson, Kent, Sigal, George
• Granted Patent: US 8,808,627 B2
• Time in Patent Office: Days
• US Class Current: 506/39
• CPC Class Codes: B01L 2300/021 Identification, e.g. bar codes
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/0851 Bottom walls
• CPC Class Codes: B01L 2300/12 Specific details about materials
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B01L 9/50 Clamping means, tongs in general F16B2/06
• CPC Class Codes: B01L 9/56 Means for indicating position of a recipient or sample in an array
• CPC Class Codes: G01N 2035/042 moved independently, e.g. by fork manipulator
• CPC Class Codes: G01N 2035/0425 Stacks, magazines or elevators for plates
• CPC Class Codes: G01N 2035/0494 Detecting or compensating piositioning errors
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 21/66 electrically excited, e.g. electroluminescence
• CPC Class Codes: G01N 21/69 specially adapted for fluids , e.g. molten metal
• CPC Class Codes: G01N 21/76 Chemiluminescence; Bioluminescence
• CPC Class Codes: Y10T 29/49155 Manufacturing circuit on or in base
• CPC Class Codes: Y10T 83/04 Processes

Abstract

Luminescence test measurements are conducted using an assay module having integrated electrodes with a reader apparatus adapted to receive assay modules, induce luminescence, preferably electrode induced luminescence, in the wells or assay regions of the assay modules and measure the induced luminescence.

Claims

1. A multi-well plate having an array of wells corresponding to a 96-well plate configuration, said array comprising one or more of the following:

a first sector having wells A1 through A4, B1 through B4, C1 through C4, and D1 through D4;

a second sector having wells A5 through A8, B5 through B8, C5 through C8, and D5 through D8;

a third sector having wells A9 through A12, B9 through B12, C9 through C12, and D9 through D12;

a fourth sector having wells E1 through E4, F1 through F4, G1 through G4, and H1 through H4;

a fifth sector having wells E5 through E8, F5 through F8, G5 through G8, and H5 through H8; and

a sixth sector having wells E9 through E12, F9 through F12, G9 through G12, and H9 through H12;

said multi-well plate having a plurality of electrodes on a top surface and a plurality of contact surfaces on a bottom surface, wherein said plurality of electrodes are electrically connected to said plurality of contact surfaces.

2. The multi-well plate of claim 1, wherein said plurality of contact surfaces are located between or adjacent said wells.

3. The multi-well plate of claim 1, wherein said plurality of electrodes comprise one or more working electrodes and one or more counter electrodes and said plurality of contact surfaces comprises one or more working contacts electrically connected to said one or more working electrodes and one or more counter contacts electrically connected to said one or more counter electrodes.

4. The multi-well plate of claim 1, wherein said contact surfaces are located at one or more of the following locations:

(i) two or more of first sector locations;

A1-B2;

A2-B3;

A3-B4;

C1-D2;

C2-D3;

C3-D4;

(ii) two or more of second sector locations;

A5-B6;

A6-B7;

A7-B8;

C5-D6;

C6-D7;

C7D8;

(iii) two or more of third sector locations;

A9-B10;

A10-B11;

A11-B12;

C9-D10;

C10-D11;

C11-D12;

(iv) two or more of fourth sector locations;

E1-F2;

E2-F3;

E3-F4;

G1-H2;

G2-H3;

G3-H4;

(v) two or more of fifth sector locations;

E5-F6;

E6-F7;

E7-F8;

G5-H6;

G6-H7;

G7-H8; and

(vi) two or more of sixth sector locations;

E9-F10;

E10-F11;

E11-F12;

G9-H10;

G10-H11;

G11-H12.

5. The multi-well plate of claim 3, wherein

(a) said working contacts are located at one or more of the following locations:

(i) one or more of first sector locations;

A1-B2;

A3-B4;

C1-D2; and

C3-D4;

(ii) one or more of second sector locations;

A5-B6;

A7-B8;

C5-D6; and

C7-D8;

(iii) one or more of third sector locations;

A9-B10;

A11-B12;

C9-D10; and

C11-D12;

(iv) one or more of fourth sector locations;

E1-F2;

E3-F4;

G1-H2; and

G3-H4;

(v) one or more of fifth sector locations;

E5-F6;

E7-F8;

G5-H6; and

G7-H8; and

(vi) one or more of sixth sector locations;

E9-F10;

E11-F12;

G9-H10; and

G11-H12; and

(b) said counter contacts are located at one or more of the following locations;

(i) one or more of first sector locations;

A2-B3 and C2-D3;

(ii) one or more of second sector locations;

A6-B7 and C6-D7;

(iii) one or more of third sector locations;

A10-B11 and C10-D11;

(iv) one or more of fourth sector locations;

E2-F3 and G2-H3;

(v) one or more of fifth sector locations;

E6-F7 and G6-H7; and

(vi) one or more of sixth sector locations;

E10-F11 and G10-H11.

11. A kit for use in the conduct of an electrode-induced luminescence assay comprising, in one or more containers, a multi-well assay plate of claim 1 and at least one electrochemiluminescent label.

12. The kit of claim 11, wherein said electrochemiluminescent label is a ruthenium or osmium-containing organometallic compound.

13. The kit of claim 12, wherein said electrochemiluminescent label comprises tris-bipyridyl-ruthenium.

14. The kit of claim 11, wherein said electrochemiluminescent label is attached to a protein.

15. The kit of claim 11, wherein said electrochemiluminescent label is attached to an antibody.

16. The kit of claim 11, wherein said electrochemiluminescent label is attached to a nucleic acid.

17. The kit of claim 11, further comprising one or more assay components, wherein at least one of said assay components is immobilized within said wells.

18. The kit of claim 17, wherein said immobilized assay component is dried within said well.

19. The kit of claim 11 wherein said kit further comprises a binding reagent which binds a component of an assay conducted in said multi-well plate.

20. The kit of claim 19, wherein said binding reagent is a protein.

21. The kit plate of claim 19, wherein said binding reagent is an antibody.

22. The kit of claim 19, wherein said binding reagent is a nucleic acid.

23. The kit of claim 22 wherein said nucleic acid binds to a complementary nucleic acid linked to an assay component.

6. A multi-well plate having an array of wells corresponding to a 384-well plate configuration, said array comprising one or more of the following:

a first sector having wells A1 through A8, B1 through B8, C1 through C8, D1 through D8, E1 through E8, F1 through F8, G1 through G8, and H1 through H8;

a second sector having wells A9 through A16, B9 through B16, C9 through C16, D9 through D16, E9 through E16, F9 through F16, G9 through G16, and H9 through H16;

a third sector having wells A17 through A24, B17 through B24, C17 through C24, D17 through D24, E17 through E24, F17 through F24, G17 through G24, and H17 through H24;

a fourth sector having wells I1 through 18, J1 through J8, K1 through K8, L1 through L8, M1 through M8, N1 through N8, O1 through O8 and P1 through P8;

a fifth sector having wells I9 through I16, J9 through I16, K9 through K16, L9 through L16, M9 through M16, N9 through N16, O9 through O16 and P9 through P16; and

a sixth sector having wells I17 through I24, J17 through J24, K17 through K24, L17 through L24, M17 through M24, N17 through 15 N24, O17 through O24 and P17 through P24;

said multi-well plate having a plurality of electrodes on a top surface and a plurality of contact surfaces on a bottom surface, wherein said plurality of electrodes are electrically connected to said plurality of contact surfaces.

7. The multi-well plate of claim 6, wherein said plurality of contact surfaces are located between or adjacent said wells.

8. The multi-well plate of claim 6, wherein said plurality of electrodes comprise one or more working electrodes and one or more counter electrodes and said plurality of contact surfaces comprises one or more working contacts electrically connected to said one or more working electrodes and one or more counter contacts electrically connected to said one or more counter electrodes.

9. The multi-well plate of claim 6, wherein said contact surfaces are located at one or more of the following locations:

(i) two or more of first sector locations;

B2-C3;

B4-C5;

B6-C7;

F2-G3;

F4-G5;

F6-G7;

(ii) two or more of second sector locations;

B10-C11;

B12-C13;

B14-C15;

F11-G11;

F12-G13;

F14-G15;

(iii) two or more of third sector locations;

B18-C19;

B20-C21;

B22-C23;

F18-G19;

F20-G21;

F22-G23;

(iv) two or more of fourth sector locations;

J2-K3;

J4-K5;

J6-K7;

N2-O3;

N4-O5;

N6-O7;

(v) two or more of fifth sector locations;

J10-K11;

J12-K13;

J14-K15;

N10-O11;

N12-O13;

N14-O15; and

(vi) two or more of sixth sector locations;

JI8-K19;

J20-K21;

J22-K23;

N18-O19;

N20-O21;

N22-O23.

10. The multi-well plate of claim 8, wherein

(a) said working contacts are located at one or more of the following locations:

(i) one or more of first sector locations;

B2-C3;

B6-C7;

F2-G3; and

F6-G7;

(ii) one or more of second sector locations;

B10-C11;

B14-C15;

F10-G11; and

F14-G15;

(iii) one or more of third sector locations;

B18-C19;

B22-C23;

F18-G19; and

F22-G23;

(iv) one or more of fourth sector locations;

J2-K3;

J6-K7;

N2-O3; and

N6-O7;

(v) one or more of fifth sector locations;

J10-K11;

J14-K15;

N10-O11; and

N14-O15; and

(vi) one or more of sixth sector locations;

J18-K19;

J22-K23;

N18-O19; and

N22-O23; and

(b) said counter contacts are located at one or more of the following locations;

(i) one or more of first sector locations;

B4-C5 and F4-G5;

(ii) one or more of second sector locations;

B12-C13 and F12-G13;

(iii) one or more of third sector locations;

B20-C21 and F20-G21;

(iv) one or more of fourth sector locations;

J4-K5 and N4-O5;

(v) one or more of fifth sector locations;

J12-K13 and N12-O13; and

(vi) one or more of sixth sector locations;

J20-K21 and N20-O21.

24. A kit for use in the conduct of an electrode-induced luminescence assay comprising, in one or more containers, a multi-well assay plate of claim 6 and at least one electrochemiluminescent label.

25. The kit of claim 6, wherein said electrochemiluminescent label is a ruthenium or osmium-containing organometallic compound.

26. The kit of claim 25, wherein said electrochemiluminescent label comprises tris-bipyridyl-ruthenium.

27. The kit of claim 6, wherein said electrochemiluminescent label is attached to a protein.

28. The kit of claim 6, wherein said electrochemiluminescent label is attached to an antibody.

29. The kit of claim 6, wherein said electrochemiluminescent label is attached to a nucleic acid.

30. The kit of claim 6, further comprising one or more assay components, wherein at least one of said assay components is immobilized within said wells.

31. The kit of claim 30, wherein said immobilized assay component is dried within said well.

32. The kit of claim 6 wherein said kit further comprises a binding reagent which binds a component of an assay conducted in said multi-well plate.

33. The kit of claim 32, wherein said binding reagent is a protein.

34. The kit plate of claim 32, wherein said binding reagent is an antibody.

35. The kit of claim 32, wherein said binding reagent is a nucleic acid.

36. The kit of claim 35 wherein said nucleic acid binds to a complementary nucleic acid linked to an assay component.