Drug Screening Using Islet Cells and Islet Cell Progenitors from Human Embryonic Stem Cells
First Claim
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1. A method of screening a factor chosen from a drug, a solvent, a peptide and a polynucleotide for its effect on a primate pancreatic islet cell precursor expressing Sox 17 comprisinga) contacting a cell culture of primate pancreatic islet cells precursors expressing Sox 17 with a factor;
- b) observing a change in the primate pancreatic islet cell precursor expressing Sox 17 after a); and
c) correlating the change in the primate pancreatic islet cell precursor expressing Sox 17 with the factor.
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Abstract
This disclosure provides a system for producing pancreatic islet cells from embryonic stem cells. Differentiation is initiated towards endoderm cells, and focused using reagents that promote emergence of islet precursors and mature insulin-secreting cells. High quality populations of islet cells can be produced in commercial quantities for use in research, drug screening, or regenerative medicine.
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Citations
18 Claims
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1. A method of screening a factor chosen from a drug, a solvent, a peptide and a polynucleotide for its effect on a primate pancreatic islet cell precursor expressing Sox 17 comprising
a) contacting a cell culture of primate pancreatic islet cells precursors expressing Sox 17 with a factor; -
b) observing a change in the primate pancreatic islet cell precursor expressing Sox 17 after a); and c) correlating the change in the primate pancreatic islet cell precursor expressing Sox 17 with the factor. - View Dependent Claims (9, 10)
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2. The method of claim, one wherein the pancreatic islet cell precursors expressing Sox 17 are human cells.
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3. The method of claim one, wherein the primate pancreatic islet cell precursor expressing Sox 17 are the in vitro progeny of a primate pluripotent stem cell.
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4. The method of claim one, wherein the factor is a drug.
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5. The method of claim one, wherein the factor is a solvent.
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6. The method of claim one, wherein the factor is a peptide.
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7. The method of claim one, wherein the factor is a polynucleotide.
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8. The method of claim one, wherein the primate pancreatic islet cell precursor expressing Sox 17 also expresses HNF3β
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11. A method of screening a factor chosen from a drug, a solvent, a peptide and a polynucleotide for its effect on a primate pancreatic islet cell or islet cell precursor expressing insulin, comprising
a) contacting a cell culture of primate pancreatic islet cells or islet cell precursors expressing insulin with a factor; -
b) observing a change in the primate pancreatic islet cell or islet cell precursor expressing insulin after a); and c) correlating the change in the primate pancreatic islet cell or islet cell precursor expressing insulin with the factor; wherein the pancreatic islet cell or islet cell precursor is the in vitro progeny of a primate pluripotent stem cell. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18)
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Specification