CENTROID MARKERS FOR IMAGE ANALYSIS OF HIGH DENISTY CLUSTERS IN COMPLEX POLYNUCLEOTIDE SEQUENCING

• US 20110065607A1
• Filed: 09/15/2009
• Published: 03/17/2011
• Est. Priority Date: 09/15/2009
• Status: Active Grant

• Current Assignee: Illumina Cambridge Limited (Illumina Incorporated)
• Original Assignee: Illumina Cambridge Limited (Illumina Incorporated)
• Inventors: Boutell, Jonathan Mark, Kersey, Alan
• Granted Patent: US 8,182,994 B2
• Time in Patent Office: Days
• US Class Current: 506/16
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/6874 involving nucleic acid arrays, e.g. sequencing by hybridisation
• CPC Class Codes: C12Q 2535/122 Massive parallel sequencing
• CPC Class Codes: C12Q 2563/107 fluorescence
• CPC Class Codes: C12Q 2565/543 characterised by the use of two or more capture oligonucleotide primers in concert, e.g. bridge amplification

Abstract

Improved compositions, methods, apparatus, and kits for high-throughput nucleic acid amplification, detection and sequencing are disclosed. A nucleic acid cluster having an identifiable center is produced by generating on a solid support an immobilized nucleic acid complement from a template, one of which comprises a detectable label; and amplifying the complement and the template to obtain a nucleic acid cluster on the support, the cluster having a substantially central location marked by the detectable label and a surrounding region comprising immobilized copies. Also disclosed are nucleotide sequence determination in nucleic acid clusters so produced, center position annotation in the clusters, assignment of sequence information to overlapping clusters, and related compositions and methods.

Claims

1. A method for producing at least one nucleic acid cluster having an identifiable center, comprising:

(I) generating, on a solid support, at least one immobilized nucleic acid complement of at least one nucleic acid template, wherein the at least one nucleic acid template or the at least one nucleic acid complement comprises a detectable label; and

(II) amplifying the at least one nucleic acid template and the at least one nucleic acid complement to obtain on the solid support at least one nucleic acid cluster, wherein each cluster comprises (a) a substantially central location comprising the at least one nucleic acid template and the at least one nucleic acid complement, and (b) a surrounding region comprising one or more immobilized copies of the at least one nucleic acid template and of the at least one nucleic acid complement, and thereby producing the at least one nucleic acid cluster having an identifiable center.

2. The method of claim 1 wherein the one or more immobilized copies of the at least one nucleic acid template and the at least one nucleic acid complement at the surrounding region lack the detectable label, and whereby the detectable label distinguishes the substantially central location from the surrounding region.

3. The method of claim 1 wherein at least 100 nucleic acid clusters each having an identifiable center are produced per square centimeter on the solid support.

4. The method of claim 1 wherein the step of amplifying is repeated one or a plurality of times under conditions and for a time sufficient for the detectable label to remain at the substantially central location in the at least one nucleic acid cluster.

5. The method of claim 1 wherein the at least one nucleic acid template is immobilized via its 5′

end to the solid support.

6. The method of claim 1 wherein (I) comprises generating at least one immobilized detectably labeled central nucleic acid complement of the at least one nucleic acid template, on the solid support, wherein the solid support comprises one or more immobilized oligonucleotide primers X, and wherein:

(a) the at least one nucleic acid template has a 5′

end and a 3′

end and comprises an oligonucleotide sequence Y at the 5′

end and an oligonucleotide sequence Z at the 3′

end,

(b) each of the one or more immobilized oligonucleotide primers X (i) is immobilized at its 5′

end to the solid support and (ii) is capable of hybridizing to the oligonucleotide sequence Z, and

(c) said at least one immobilized detectably labeled central nucleic acid complement comprises (i) an extension of one of the immobilized oligonucleotide primers X, and (ii) at least one detectable label.

7. The method of claim 6 wherein the oligonucleotide sequence Z is complementary to the oligonucleotide sequence Y and the immobilized oligonucleotide primer X comprises a sequence that is substantially identical to oligonucleotide sequence Y.

8. The method of claim 6 wherein the solid support comprises a first and a second immobilized oligonucleotide primer X which are different from each other, wherein the oligonucleotide sequence Z can hybridize to the first immobilized oligonucleotide primer X and the second immobilized oligonucleotide primer X has a sequence that is substantially identical to oligonucleotide sequence Y.

9. The method of claim 6 wherein generating comprises:

(a) initiating, in the presence of one or more labeled nucleotides that each comprise a detectable label, a primer extension reaction on the at least one nucleic acid template whereby the one or more immobilized oligonucleotide primers X are extended by incorporation, into the immobilized detectably labeled central nucleic acid complement, of one or more of said labeled nucleotides that each comprise a detectable label;

(b) stopping the primer extension reaction and removing therefrom unincorporated labeled nucleotides; and

(c) re-initiating the primer extension reaction in the presence of unlabeled nucleotides.

10. The method of claim 9 wherein steps 9(a) and 9(b) are repeated one or a plurality of times, and wherein a single nucleotide comprising a detectable label is incorporated into the central nucleic acid complement at each step 9(a).

11. The method of claim 1 wherein amplifying comprises thermocycling amplification.

12. The method of claim 1 wherein amplifying comprises isothermal amplification.

13. The method of claim 1 further comprising recording a center position in the nucleic acid cluster by detecting the at least one detectable label and therefrom identifying the substantially central location in the cluster.

14. The method of claim 13 further comprising determining a nucleotide sequence in the at least one nucleic acid cluster by performing one or a plurality of sequencing steps on at least one of (i) the at least one nucleic acid template or an immobilized copy thereof, and (ii) the immobilized detectably labeled central nucleic acid complement or an immobilized copy thereof.

15. The method of claim 14 wherein each sequencing step comprises incorporating a labeled sequencing nucleotide into the at least one nucleic acid cluster;

and subsequently detecting said incorporated labeled sequencing nucleotide in the at least one nucleic acid cluster.

16. The method of claim 14 wherein full or partial nucleotide sequences are determined simultaneously in more than one nucleic acid cluster, each of said more than one clusters having an identified substantially central location.

17. The method of claim 15 wherein:

(i) full or partial nucleotide sequences are determined simultaneously in more than one nucleic acid cluster, each of said more than one clusters having an identifiable center,

(ii) each sequencing step further comprises detecting a position of the incorporated labeled sequencing nucleotide on the solid support, and

(iii) a determined sequence can be assigned to a discrete nucleic acid cluster when there is a substantial correlation between (i) the recorded center position of each nucleic acid cluster and (ii) the position of each incorporated labeled sequencing nucleotide on the solid support that is detected in each sequencing step.

18. The method of claim 17 wherein for each of two or more overlapping nucleic acid clusters, the determined nucleotide sequence is assigned to one discrete nucleic acid cluster.

19. The method of claim 16 wherein the step of recording is performed prior to amplifying of step (II).

20. The method of claim 16 wherein the step of recording is performed after amplifying of step (II).

21. The method of claim 17 wherein the one or more detectable labels are not detectably discrete from any incorporated labeled sequencing nucleotide detected at each sequencing step.

22. The method of claim 14 wherein the step of recording is performed at each sequencing step.

23. The method of claim 17 wherein the one or more detectable labels are detectably discrete from each of the incorporated labeled sequencing nucleotides detected at each sequencing step.

24. A method for annotating a center position of at least one nucleic acid cluster, comprising:

(I) generating, on a solid support, at least one immobilized nucleic acid complement of at least one nucleic acid template, wherein the at least one nucleic acid template or the at least one nucleic acid complement comprises a detectable center label; and

(II) amplifying the at least one nucleic acid template and the at least one nucleic acid complement to obtain on the solid support at least one nucleic acid cluster, wherein each cluster comprises (a) a substantially central location comprising the at least one nucleic acid template and the at least one nucleic acid complement, and (b) a surrounding region comprising one or more immobilized copies of the at least one nucleic acid template and of the at least one nucleic acid complement; and

(III) recording a center position in the at least one nucleic acid cluster by detecting the detectable center label to identify the substantially central location in the at least one nucleic acid cluster as the center position, and thereby annotating the center position of the at least one nucleic acid cluster.

25. A method for assigning nucleotide sequence information to at least one of two or more nucleic acid clusters that overlap on a solid support, comprising:

(a) providing two or more nucleic acid clusters that detectably overlap on a solid support, each of said clusters comprising (i) a substantially central location comprising an immobilized central nucleic acid having a detectable center label, and (ii) a surrounding region comprising one or more immobilized copies of the immobilized central nucleic acid, wherein the detectable center label distinguishes the substantially central location from the surrounding region;

(b) detecting the detectable center label in each of said overlapping nucleic acid clusters and therefrom identifying in each cluster a first position for the substantially central location on the solid support;

(c) determining a nucleotide sequence in at least one cluster of said overlapping nucleic acid clusters by performing one or a plurality of sequencing steps thereupon, thereby identifying a second position for the at least one cluster on the solid support; and

(d) assigning a nucleotide sequence determined in (c) to a discrete nucleic acid cluster by substantially correlating (i) the first position for the substantially central location of (b) with (ii) the second position for the at least one cluster on the solid support of (c), and thereby assigning nucleotide sequence information to at least one of two or more nucleic acid clusters that overlap on the solid support.

26. A composition comprising:

(a) a solid support; and

(b) one or more nucleic acid clusters each comprising a plurality of nucleic acids having the same sequence and being immobilized to the solid support, wherein each nucleic acid cluster comprises an identifiable center comprising a first subpopulation of the plurality of nucleic acids that is surrounded by a second subpopulation of the nucleic acids, wherein each of the nucleic acids in the first subpopulation comprises one or more detectable center labels that distinguish the nucleic acids in the first subpopulation from the nucleic acids in the second population.