Method and Assay for Detection of Residues
First Claim
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1. A lateral flow assay for detecting an analyte in a solution, the assay comprising:
- (a) a binder configured for generating a detectable signal, wherein the binder can combine with an analyte from the solution to form a binder-analyte complex;
(b) a test strip configured to allow a test solution to flow, wherein the test solution contains either or both a binder and a binder-analyte complex, the test strip comprising;
(i) a test zone having immobilized thereon a test zone capture agent that, when the binder has not formed a binder-analyte complex, captures the binder,(ii) a single control zone having a plurality of control zone capture agents, each of the plurality of control zone capture agents having different affinity to the binder and each able to capture the binder, whether or not the binder has formed a binder-analyte complex,wherein capture of the binder at either the test zone or control zone results in a detectable signal and wherein a greater signal in the control zone as compared to the test zone indicates a positive result.
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Abstract
Embodiments described herein include methods and assays for detecting an analyte in a sample using a plurality of control zone capture agents. Some embodiments include detection of multiple analytes in a sample utilizing a plurality of analyte binders and a control zone containing multiple control zone capture agents. In some embodiments, the multiple control zone capture agents capture a plurality of binders within one control zone. Test results are determined by comparison of the control zone signal to a test zone signal.
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Citations
38 Claims
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1. A lateral flow assay for detecting an analyte in a solution, the assay comprising:
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(a) a binder configured for generating a detectable signal, wherein the binder can combine with an analyte from the solution to form a binder-analyte complex; (b) a test strip configured to allow a test solution to flow, wherein the test solution contains either or both a binder and a binder-analyte complex, the test strip comprising; (i) a test zone having immobilized thereon a test zone capture agent that, when the binder has not formed a binder-analyte complex, captures the binder, (ii) a single control zone having a plurality of control zone capture agents, each of the plurality of control zone capture agents having different affinity to the binder and each able to capture the binder, whether or not the binder has formed a binder-analyte complex, wherein capture of the binder at either the test zone or control zone results in a detectable signal and wherein a greater signal in the control zone as compared to the test zone indicates a positive result. - View Dependent Claims (3, 4, 5, 7, 8, 11, 12, 27)
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2. (canceled)
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6. (canceled)
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9. (canceled)
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10. (canceled)
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13-26. -26. (canceled)
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28. A lateral flow assay for detecting a plurality of analytes in a solution, the assay comprising:
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(a) a plurality of binders configured for generating a detectable signal, wherein each of the binders are capable of combining with an analyte from the solution to form a binder-analyte complex; (b) a test strip configured to allow a test solution to flow, wherein the test solution contains either or both a binder and a binder-analyte complex, the test strip comprising; (i) a plurality of test zones, each test zone having immobilized thereon a test zone capture agent that, when the binder has not formed a binder-analyte complex, captures at least one of the binders, (ii) a single control zone having a plurality of control zone capture agents, each of the plurality of control zone capture agents having different affinity and each able to capture at least one of the binders, whether or not the binder has formed a binder-analyte complex, wherein capture of a binder at either of the test zones or control zone results in a detectable signal and wherein a greater signal in the control zone as compared to any one of the test zones indicates a positive result. - View Dependent Claims (29, 30, 31, 32, 33, 34, 35, 36, 37)
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38. A lateral flow assay for detecting one or more beta-lactam antibiotics in a solution, the assay comprising:
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(a) a beta lactam binder configured for generating a detectable signal, wherein the binder can combine with a beta lactam antibiotic from the solution to form a binder-analyte complex; (b) a test strip configured to allow a test solution to flow, wherein the test solution contains either or both a binder and a binder-analyte complex, the test strip comprising; (i) a test zone having immobilized thereon a test zone capture agent the test zone capture agent comprising a beta lactam that, when the binder has not formed a binder-analyte complex, captures the binder, (ii) a single control zone having a plurality of control zone capture agents, each of the plurality of control zone capture agents having different affinity to the binder and each able to capture the binder, whether or not the binder has formed a binder-analyte complex, wherein capture of the binder at either the test zone or control zone results in a detectable signal and wherein a greater signal in the control zone as compared to the test zone indicates a positive result.
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Specification